Maize event nCX-1 was produced by Agrobacterium tumefaciens-mediated transformation of maize with the vector 1300-N-Z1-CP4.

The T-DNA of the transformation vector 1300-N-Z1-CP4 has two gene cassettes:

• the N-Z1 cassette (a cytochrome P450 gene from Bermuda grass Cynodon dactylon) confers tolerance to multiple herbicides: acetolactate synthase (ALS) inhibitor herbicides nicosulfuron and penoxsulam; phydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide mesotrione; synthetic auxin herbicide dicamba; photosynthesis inhibitor bentazon; nicosulfuron, flazasulfuron, and 2,4-dichlorophenoxyacetic acid (2,4-D),
• a modified cp4-epsps expression cassette (CS-CP4epsps-RHIRD) which encodes for the 5-enolpyruvylshikimic acid-3-phosphate synthase (EPSPS) protein to confer resistance to glyphosate herbicides,

The Patent states that the invention provides a transgenic maize event nCX-1 with single copy insertion of exogenous genes, good heritage stability, high resistance to excellent characters such as flazasulfuron, nicosulfuron, dimethyltetrachloro, 2,4-D and glyphosate.

Sources: Zheng et al. (2022) Patent CN116103423A/en