GMO molecular characterization details

GMO detailed information

GMO:
J3
Alias:
UID:
SPS-ØØØJ3-4
Tradename:

Innate
Companies/Institutions:
J.R. Simplot
Species:
Solanum tuberosum
(potato)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert_J3
Comment:	insert_J3 consists of a nearly full-length T-DNA with a small deletion at the Left Border site ("insert order" No. 9-24) flanked by an additional Asn1/Ppo5 ("insert order" No. 1-8) and partial phL/R1 ("insert order" No. 25-30) cassette from pSIM1278. according to: USDA-APHIS petitions 13-022-01p and 16-064-01p
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	P-Agp-SOLTU	Solanum tuberosum	-			Truncated; not functional.
2	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.
3	CS-Asn1_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in sense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene.
4	CS-ppo5_RNAi_s-SOLVR	Solanum verrucosum	-	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in sense orientation . Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5.
5	V-intervening-SOLTU	Solanum tuberosum	-
6	CS-ppo5_RNAi_as-SOLVR	Solanum verrucosum	-	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in antisense orientation. Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5.
7	CS-Asn1_RNAi_as-SOLTU	Solanum tuberosum	-	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in antisense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene.
8	P-Agp-SOLTU	Solanum tuberosum	-			Truncated.
9	P-Agp-SOLTU	Solanum tuberosum	-			One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
10	CS-Asn1_RNAi_as-SOLTU	Solanum tuberosum	-	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in antisense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene; generates with the corresponding copy in sense orientation a double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
11	CS-ppo5_RNAi_as-SOLVR	Solanum verrucosum	-	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in antisense orientation. Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5 gene; generates with the corresponding copy in sense orientation a double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
12	V-intervening-SOLTU	Solanum tuberosum	-
13	CS-ppo5_RNAi_s-SOLVR	Solanum verrucosum	-	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in sense orientation . Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5 gene; generates with the corresponding copy in antisense orientation a double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
14	CS-Asn1_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in sense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene; generates with the corresponding copy in antisense orientation a double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
15	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
16	P-Agp-SOLTU	Solanum tuberosum	-			One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
17	CS-phl_RNAi_as-SOLTU	Solanum tuberosum	-	Fragment of promoter of the phosphorylase-L (phl) gene from Solanum tuberosum in antisense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato Phl gene; generates with the corresponding copy in sense orientation a double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
18	CS-R1_RNAi_as-SOLTU	Solanum tuberosum	-	Fragment of promoter of the dikinase r1 gene from Solanum tuberosum in antisense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato R1 gene; generates with the corresponding copy in sense orientation a double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
19	V-intervening-SOLTU	Solanum tuberosum	-
20	CS-R1_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of promoter of the dikinase r1 gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato R1 gene; generates with the corresponding copy in antisense orientation a double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
21	CS-phl_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of promoter of the phosphorylase-L (phl) gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato Phl gene; generates with the corresponding copy in antisense orientation a double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
22	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
23	V-MCS-SYNTH	Synthetic	-
24	V-RB-SYNTH	Synthetic	-
25	V-RB-SYNTH	Synthetic	-
26	V-MCS-SYNTH	Synthetic	-
27	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.
28	CS-phl_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of promoter of the phosphorylase-L (phl) gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content
29	CS-R1_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of promoter of the dikinase r1 gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content
30	V-intervening-SOLTU	Solanum tuberosum	-			Truncated.

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

USDA-APHIS No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No

Transformation Vectors

Name:	vector_pSIM1278
Comment:	The plasmid pSIM1278 is a binary transformation vector consisting of two parts: the vector backbone and the DNA insert. The backbone section contains well-characterized bacterial origins of replication from plasmids pVS1 and pBR322, and the nptII gene for bacterial resistance to kanamycin. An expression cassette comprising the Agrobacterium ipt gene preceded by the polyubiquitin (Ubi7) promoter (GenBank accession no. U26831.1) and followed by the Ubi3 terminator was introduced as a 2.6-kb SacII fragment into the vector backbone (Garbarino and Belknap 1994). Only the DNA insert (region between left border and right border) is shown. Source: Described in USDA-APHIS petition 13-022-01p (p29-31) and petition 15-140-01p (p21-22). Entered according to petition 15-140-01p (position of the genetic elements amended; more intervening sequences described, same order of the elements). https://www.aphis.usda.gov/brs/aphisdocs/15_14001p.pdf
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-SYNTH	Synthetic	25	1-25
2	V-MCS-SYNTH	Synthetic	168	26-193			Includes KpnI restriction site.
3	P-Agp-SOLTU	Solanum tuberosum	2260	194-2453			One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
4	CS-Asn1_RNAi_as-SOLTU	Solanum tuberosum	405	2454-2858	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in antisense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
5	CS-ppo5_RNAi_as-SOLVR	Solanum verrucosum	144	2859-3002	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in antisense orientation. Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
6	V-intervening-SOLTU	Solanum tuberosum	163	3003-3165			Includes XbaI restriction site.
7	CS-ppo5_RNAi_s-SOLVR	Solanum verrucosum	144	3166-3309	Fragment of the polyphenol oxidase 5 (ppo5) gene from Solanum verrucosum in sense orientation . Thygesen, P.W. et al. (1995). Plant Physiol. 109:525-531.	Reduced black spot bruising	3' untranslated sequence of Ppo5; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
8	CS-Asn1_RNAi_s-SOLTU	Solanum tuberosum	406	3310-3715	Fragment of the asparagine synthetase (asn1) gene from Solanum tuberosum in sense orientation. ASN1 enzyme is involved in synthesis of free asparagine. Chawla, R. et al. (2012). Plant Biotechnol. J., 10: 913-924. doi: 10.1111/j.1467-7652.2012.00720.x	Reduced acrylamide potential	Fragment of the Asn1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
9	V-intervening-SOLTU	Solanum tuberosum	6	3716-3721			EcoRI restriction site.
10	P-gbss-SOLTU	Solanum tuberosum	686	3722-4407	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
11	V-intervening-SOLTU	Solanum tuberosum	16	4408-4423			Includes SpeI and KpnI restritcion sites.
12	P-Agp-SOLTU	Solanum tuberosum	2260	4424-6683			One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
13	CS-phl_RNAi_as-SOLTU	Solanum tuberosum	509	6684-7192	Fragment of promoter of the phosphorylase-L (phl) gene from Solanum tuberosum in antisense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato Phl gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
14	CS-R1_RNAi_as-SOLTU	Solanum tuberosum	532	7193-7724	Fragment of promoter of the dikinase r1 gene from Solanum tuberosum in antisense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato R1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
15	V-intervening-SOLTU	Solanum tuberosum	264	7725-7988			Includes PstI restriction site.
16	CS-R1_RNAi_s-SOLTU	Solanum tuberosum	532	7989-8520	Fragment of promoter of the dikinase r1 gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato R1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
17	CS-phl_RNAi_s-SOLTU	Solanum tuberosum	509	8521-9029	Fragment of promoter of the phosphorylase-L (phl) gene from Solanum tuberosum in sense orientation. Rommens, C. et al. (2007). J. Agric. Food Chem., 54, 9882-7. doi: 10.1021/jf062477l	Altered sugars content	Fragment of promoter for the potato Phl gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
18	P-gbss-SOLTU	Solanum tuberosum	924	9030-9953	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30.		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
19	V-MCS-SYNTH	Synthetic	170	9954-10123			Includes SacI restriction site.
20	V-RB-SYNTH	Synthetic	25	10124-10148

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No	10148

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Related DNA Sequences

Detection Methods

Reference Materials

Authorisation

Literature reference