GMO molecular characterization details

GMO detailed information

GMO:
GE-Pusa DST rice 1
Alias:

GE rice, gene editing
UID:
Tradename:
Companies/Institutions:
Indian Council of Agricultural Research - Indian Institute of Rice Research (ICAR-IIRR - Hyderabad, India)
Species:
Oryza sativa
(rice)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Description:

GE-Pusa DST rice 1 was developed using CRISPR/Cas9. The drought and salt tolerance (dst) gene was edited in rice variety Maruteru 1010 (MTU1010) resulting in higher yields in high salinity and alkaline soils.

Source: Press release https://www.drishtiias.com/daily-updates/daily-news-analysis/genome-edited-rice-varieties

Links regarding to this GMO:

Press release https://www.drishtiias.com/daily-updates/daily-news-analysis/genome-edited-rice-varieties,

ISAAA https://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=21330,

ICAR https://icar.org.in/en/union-agriculture-minister-shri-shivraj-singh-chouhan-announces-two-genome-edited-rice-varieties

Transformation / Modification technique:

CRISPR/Cas

Inserts / Modification

Name:	edited_Pusa DST rice 1
Comment:
Map:

Genetic Elements:

Reference: others

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
	CS-dst_genome edited-ORYSA	Oryza sativa		-	Gene coding for the drought and salt tolerance (dst) protein in rice. Huang, X.-Y. and Chao, D.-Y. et al. (2009). Genes and Dev, 23, 1805-1817. doi: 10.1101/gad.1812409.	Salinity tolerance	Endogenous gene; no additional information on the modification available

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

others No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	others	No

Transformation Vectors

No data found

Detection Methods

7 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-TAX-OS-002	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006).	in-house validation	unknown	1
QL-TAX-OS-003	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005)	ring trial validation	national standard	1
QL-TAX-OS-006	taxon-specific	Oryza sativa	Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).	ring trial validation	unknown	1
QT-TAX-OS-002	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016)	ring trial validation	EU reference method	1
QT-TAX-OS-003	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009)	ring trial validation	unknown	1
QT-TAX-OS-017	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-TAX-SPS primer f/SPS primer r	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1)	ring trial validation	ISO standard	1

Reference Materials

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Literature reference

Content Types

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European GMO Initiative for a Unified Database System

GMO detailed information

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Species:

Traits:

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