GMO molecular characterization details

GMO detailed information

GMO:
A5547-127
Alias:

LL55
UID:
ACS-GMØØ6-4
Tradename:

LibertyLink
Companies/Institutions:
Bayer CropScience, AgrEvo
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_A5547-127
Comment:
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	O-flanking DNA-HOST		-
2	V-bla-ECOLX	Escherichia coli	-			non-functional fragment of the 5´part of CS-bla-ECOLX
3	V-pUC19-SYNTH	Synthetic	-
4	P-35S-CaMV	Cauliflower mosaic virus	-			element length: 543 bp
5	V-MCS-SYNTH	Synthetic	-			element length: 8 bp
6	CS-pat-STRVR	Streptomyces viridochromogenes	-	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37.	Glufosinate tolerance	element length: 552 bp
7	V-MCS-SYNTH	Synthetic	-			element length: 18 bp
8	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888.		element length: 203 bp
9	V-pUC19-SYNTH	Synthetic	-
10	V-bla-ECOLX	Escherichia coli	-			non-functional fragment of the 3´part of CS-bla-ECOLX
11	O-flanking DNA-HOST		-

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	application documents	No
DM079060	NCBI - GenBank	Yes	810	Elite event A5547-127 and methods and kits for identifying such event in biological samples
DM079061	NCBI - GenBank	Yes	1880	Elite event A5547-127 and methods and kits for identifying such event in biological samples.
GQ497217	NCBI - GenBank	Yes	3210	Glycine max transgenic GMO cassette genomic sequence.
KJ608130	NCBI - GenBank	Yes	530	Glycine max transgenic line A5547-127 promoter region.
RIKILT_A5547-127_event	Wageningen Food Safety Research (WFSR)	Yes	75	Amplicon sequence of A5547-127 for event-specific method QT-EVE-GM-007 without probe

Detection Methods

28 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-005	construct-specific	40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP32316, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2	Qualitative conventional PCR method for detection of maize event T25 (ISO 21569)	ring trial validation	ISO standard	2
QL-CON-00-011	construct-specific	40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, B0050-027, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DP32316, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3).	ring trial validation	ISO standard	3
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-021	element-specific	CS-pat-STRVR	Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-025	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.	ring trial validation	EU reference method	3
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	3
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	3
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-005	construct-specific	40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP32316, DP62151, DP910521, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2	Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002)	ring trial validation	ISO standard	2
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-002	element-specific	CS-pat-STRVR	Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)	ring trial validation	unknown	2
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	3
QT-EVE-GM-007	event-specific	A5547-127	Quantitative real-time PCR (TaqMan) method for detection of soybean event A5547-127 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Catalogue number	Source	Value	Type	Certified	Available
5211512801	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no
5211521401	Eurofins GeneScan	10,000 cp/µl	genomic DNA	no	no
AOCS 0707-A7	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0707-A8	AOCS	<0.005 % (m/m)	genomic DNA	yes	no
AOCS 0707-C3	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0707-C4	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0707-C7	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0707-C8	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0707-C9	AOCS	>99.99 % (m/m)	genomic DNA	yes	yes

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Commission decision number	Expiry date
1	EFSA-GMO-RX-020	Glycine max (soybean)	A5547-127	food feed cultivation other	BASF Agricultural Solutions Seed US	(EU) 2023/415	21.02.2033
2	EFSA-GMO-NL-2008-52	Glycine max (soybean)	A5547-127	food feed cultivation other	BASF Agricultural Solutions Seed US	2012/81/EU	09.02.2022

Citation	Publication year	Publishing authority	Publication type	Content types
Commission implementing decision (EU) 2023/415 of 22 February 2023 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified soybean A5547-127 (ACS-GMØØ6-4) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2023) 1126)	2023	EU	EU Commission Decision	decision document
Assessment of genetically modified soybean A5547‐127 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐RX‐020)	2022	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Request for Renewal of the Authorisation of the genetically modified herbicide tolerant soybean A5547-127 for food and feed uses, and import and processing, in accordance with articles 11 and 23 of Regulation (EC) No 1829/2003, EFSA-GMO-RX-020, Part III - Summary	2020	EFSA	application summary	application summary
Horizontal methods for molecular biomarker analysis — Methods of analysis for the detection of genetically modified organisms and derived products — Part 3: Construct-specific real-time PCR method for detection of P35S-pat-sequence for screening for genetically modified organisms (ISO/TS 21569-3)	2020	ISO	other document	detection
Huber, I. et al. (2013). Development and Validation of Duplex, Triplex, and Pentaplex Real-Time PCR Screening Assays for the Detection of Genetically Modified Organisms in Food and Feed. J. Agric. Food Chem. 61, 10293–10301	2013	Research group	peer-reviewed article	detection
COMMISSION IMPLEMENTING DECISION of 10 February 2012 authorising the placing on the market of products containing, consisting of, or produced from genetically modified soybean A5547-127 (ACS-GMØØ6-4) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2012) 691) (2012/81/EU)	2012	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-NL-2008-52) for the placing on the market of the genetically modified soybean A5547-127 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience	2011	EFSA	EFSA overall opinion	risk assessment document
PART II SUMMARY REQUEST FOR AUTHORIZATION OF GM FOOD AND GM FEED IN ACCORDANCE WITH ATICLES 5 AND 17 OF REGULATION (EC) NO. 1829/2003 GLUFOSINATE AMMONIUM-TOLERANT SOYBEAN TRANSFORMATION EVENT A5547-127	2011	EFSA	application summary	application summary
Scientific Opinion on application (EFSA-GMO-NL-2008-52) for the placing on the market of herbicide tolerant genetically modified soybean A5547-127 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience	2011	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Delobel C., Bogni A., Mazzara M., Van den Eede G.; Event-specific Method for the Quantification of Soybean Line A5547-127 Using Real-time PCR - Validation Report and Protocol Online Publication (2009)	2009		EU reference method protocol	detection

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference