Description:
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Oilseed rape GT73 was developed by the Agrobacterium tumefaciens-mediated transformation of the oilseed rape cultivar Westar with plasmid vector PV-BNGT04.
PV-BNGT04 included one transfer DNA (T-DNA), which contained two expression cassettes between the right and left borders:
The goxv247 expression cassette consisted of the 34S promoter from Figwort mosaic virus (FMV); the CTP1 chloroplast transit peptide-encoding sequence derived from the Arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (RuBisCO) small subunit 1A gene; the goxv247 synthetic coding sequence encoding the glyphosate oxidoreductase variant GOXv247; and the 3’ untranslated region of the pea RuBisCO small subunit E9 gene, which serves as a transcription terminator. The GOXv247 protein expressed in oilseed rape GT73 was developed from the GOX protein of Ochrobactrum anthropic strain LBAA and differs from the wild-type variant in only three amino acid positions out of 431.
The CP4-epsps expression cassette consisted of the same FMV promoter used in the goxv247 expression cassette; the CTP2 chloroplast transit peptide-encoding sequence of the epsps gene from A. thaliana; the CP4 epsps coding sequence from A. tumefaciens strain CP4; and the 3’ untranslated region of the pea RuBisCO small subunit E9 gene, which serves as a transcription terminator.
GT73 is tolerant to glyphosate because the produced GOXv247 protein effectively inactivates the herbicide and enables growth when GT73 plants are treated with glyphosate and the CP4 EPSPS gene confers a high tolerance to glyphosate.
The molecular characterisation data established that the oilseed rape GT73 contains one copy of an intact goxv247 expression cassette and a CP4 epsps cassette in a single locus. No other parts of the plasmid used for transformation are present in oilseed rape GT73.
Genetic stability of the inserted DNA was studied over three generations of oilseed rape GT73 by Southern blot analysis.
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Links regarding to this GMO:
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USDA_APHIS petition http://www.aphis.usda.gov/brs/aphisdocs2/98_21601p_com.pdf,
ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=10,
BCH Record ID 14795 http://bch.cbd.int/database/record.shtml?documentid=14795
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Transformation / Modification technique:
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Agrobacterium-induced
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