PF967 was developed using Agrobacterium-mediated transformation of plant tissue of the flax cultivar Norlin. It was engineered to be tolerant to sulfonylurea residues in the soil by transformation with:

• a modified acetolactate synthase (als) gene from Arabidopsis thaliana.

In addition two selectable markers were introduced:

• the neomycin phosphotransferase II (nptII) gene from Escherichia coli
• the nopaline synthase (nos) gene from Agrobacterium tumefaciens.

Ampicillin and spectinomycin antibiotic resistance selectable markers from E. coli were introduced into the plant but are not expressed.

Southern DNA restriction analyses show that there are two insertions of the complete T-DNA at different loci, that the transferred DNA does not include functional bacterial DNA outside the T-DNA and that the T-DNA is stable over several seed generations.