GMO molecular characterization details

GMO detailed information

GMO:
CTB141175/01-A
Alias:

CTC 20 BT, CTC175-A, CTC20 Bt
UID:
CTC-14117-4
Tradename:
Companies/Institutions:
Centro de Tecnologia Canavieira (CTC)
Species:
Saccharum officinarum
(sugar cane, sugarcane)
Traits:
EU authorisation:

no Reference materials could be found
No results found.

General Information

Inserts / Modification

Name:	insert_CTB141175/01-A
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order and copy number of genetic elements in the insert is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-pepc-MAIZE	Zea mays		-	Promoter of the peroxidase phosphoenolpyruvate carboxylase (PEPC) gene. Hudspeth and Grula (1989) Plant Mol Biol, 12(5):579-589		length: 2.30 kb
2	CS-cry1Ab-BACTU	Bacillus thuringiensis		-	codon optimized gene coding for a cry1Ab delta-endotoxin from B. thuringiensis.	Lepidoptera resistance	length: 1.90 kb
3	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		length: 0.30 kb
4	P-ubi1-MAIZE	Zea mays		-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689		length: 2.00 kb
5	CS-nptII-ECOLX	Escherichia coli		-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance	length: 0.80 kb
6	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		length: 0.30 kb

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Detection Methods

15 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-cry1A 4-5'/cry1A 4-3'	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-020	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-00-003	element-specific	CS-cry1Ab-BACTU	Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570)	ring trial validation	ISO standard	1

Authorisation

Literature reference