GMO molecular characterization details

GMO detailed information

GMO:
SBVR111
Alias:

GM RZ 13
UID:
Tradename:
Companies/Institutions:
Syngenta
Species:
Beta vulgaris
(sugar beet)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

Inserts / Modification

Name:	insert_SBVR111
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-ubi3-ARATH	Arabidopsis thaliana		-	Promoter of the Ubiquitin-3 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906
2	CS-RdRp_RNAi_s-BNYVV	Beet necrotic yellow vein virus		-	Fragment of the RNA-1 gene in sense orientation. Bouzoubaa et al. (1987). J gen Virol, 68, 615-626	Beet necrotic yellow vein virus resistance	fragment of RNA-dependant RNA polymerase of RNA-1 of BNYVV (Genbank accession NC_003514) that confers resistance to rhizomania which is a disease that is induced by BNYVV if an inverted repeat is expressed.
3	CS-RdRp_RNAi_as-BNYVV	Beet necrotic yellow vein virus		-	Fragment of the RNA-1 gene from Beet necrotic yellow vein virus in antisense orientation. Bouzoubaa et al. (1987). J gen Virol, 68, 615-626	Beet necrotic yellow vein virus resistance	fragment of RNA-dependant RNA polymerase of RNA-1 of BNYVV (Genbank accession NC_003514) that confers resistance to rhizomania which is a disease that is induced by BNYVV if an inverted repeat is expressed.
4	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
5	P-hsp80-BRAOL	Brassica oleracea		-	Promoter region of the heat shock protein 80 (hsp80) gene. Brunke and Wilson (1993) EP0 559 603 A2
6	CS-pmi-ECOLX	Escherichia coli		-	Phosphomannose isomerase. Reed et al (2001). In Vitro Cell Dev Biol Plant, 37, 127-132	Mannose metabolism
7	T-Cauliflower mosaic virus			-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Detection Methods

10 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-023	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-TAX-BV-013	taxon-specific	Beta vulgaris	Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014)	ring trial validation	EU reference method	1

Authorisation

Literature reference