GMO molecular characterization details

GMO detailed information

GMO:
98140
Alias:

DP98140, Event 98140
UID:
DP-Ø9814Ø-6
Tradename:

Optimum GAT
Companies/Institutions:
DuPont, Pioneer Hi-Bred International
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_98140 (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the transformation vector, the binary vector PHP24279.
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens		-
2	T-pinII-SOLTU	Solanum tuberosum		-	3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence.
3	CS-gat-BACLI	Bacillus licheniformis		-	Glyphosate-N-Acteyltransferase gene. Castel et al. (2004). Science, 304, 1151-1154	Glyphosate tolerance
4	I-1_ubi1-MAIZE	Zea mays		-
5	L-ubi-MAIZE	Zea mays		-
6	P-ubi1-MAIZE	Zea mays		-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
7	E-35S-CaMV	Cauliflower mosaic virus		-
8	E-35S-CaMV	Cauliflower mosaic virus		-
9	E-35S-CaMV	Cauliflower mosaic virus		-
10	P-ahas-MAIZE	Zea mays		-	Promoter of the acetohydroxy acid synthase gene. Dietrich et al. (1996) WO1996007746A1
11	CS-ahas-MAIZE	Zea mays		-	Acetohydroxy acid synthase gene (mutated). Lawrence et al. (1992). Plant Mol Biol 18, 1185-1187	Sulfonylurea tolerance
12	T-pinII-SOLTU	Solanum tuberosum		-	3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence.
13	V-Flanking Ti-nos-RHIRD	Agrobacterium tumefaciens		-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the nopaline type; sequence located in the promoter of nopaline synthase.
14	V-RB-RHIRD	Agrobacterium tumefaciens		-

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	application documents	No
GM746248	NCBI - GenBank	Yes	9423	Sequence 48 from Patent WO2008112019, sequence of event Event98140 (including amplicon for method QT-EVE-ZM-021)
RIKILT_Event_98140_event	Wageningen Food Safety Research (WFSR)	Yes	80	Amplicon sequence of Event 98140 for event-specific method QT-EVE-ZM-021 without probe

Detection Methods

18 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-gat/T-pinII-F/gat/T-pinII-R	construct-specific	61061, 73496, 98140, DP356043	Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016)	in-house validation	national standard	3
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-ZM-021	event-specific	98140	Quantitative real-time PCR (TaqMan) method for detection of maize event 98140 (EU-RL GMFF GMOMETHODS database as January 10, 2014)	ring trial validation	EU reference method	3
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-008	taxon-specific	Zea mays	Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-zSSIIb-3F/zSSIIb-4R	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)	unknown	unknown	1

Reference Materials

Authorisation

Literature reference