QL-CON-00-014 |
construct-specific |
1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4) |
ring trial validation |
ISO standard |
1 |
QL-ELE-npt 1-5'/npt 1-3' |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013). |
in-house validation |
none |
1 |
QL-ELE-P-nos-F/P-nos-R |
element-specific |
P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-002 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-003 |
element-specific |
CS-nptII-ECOLX, V-nptII-ECOLX |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) |
ring trial validation |
national standard |
1 |
QL-ELE-00-004 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-005 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-006 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-007 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
ring trial validation |
national standard |
1 |
QL-ELE-00-008 |
element-specific |
P-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-009 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-011 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-012 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-013 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-016 |
element-specific |
CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU |
Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-017 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-018 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
element-specific |
T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
ring trial validation |
none |
1 |
QT-ELE-P35S 1-5'/P35S 1-3' |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) |
ring trial validation |
national standard |
1 |
QT-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) |
ring trial validation |
unknown |
1 |
QT-ELE-00-004 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
ring trial validation |
ISO standard |
1 |
QT-TAX-GH-015 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-016 |
taxon-specific |
Gossypium hirsutum |
Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-018 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-019 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-020 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-GH-021 |
taxon-specific |
Gossypium hirsutum |
Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |