QL-CON-00-005 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QL-CON-00-008 |
construct-specific |
2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-CON-00-011 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, Bt10 maize, Bt11, DAS1507, DAS59122, DAS59132, DBN9004, DHA, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, MZHG0JG, MZIR098, SYHT0H2, T120-7, T14, T25, T45, Topas 19/2 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the 35S promoter and the phosphinothricin N-acetyltransferase (pat) gene (ISO 21569-3). |
ring trial validation |
ISO standard |
1 |
QL-CON-00-013 |
construct-specific |
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6 |
Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015). |
ring trial validation |
EU reference method |
1 |
QL-ELE-AgroBS1 F/AgroBS1 R |
element-specific |
V-Flanking Ti-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-ELE-AgroBS2 F/AgroBS2 R |
element-specific |
V-Flanking Ti-ocs-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II.
Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176) |
ring trial validation |
national standard |
1 |
QL-ELE-AINT 2-5'/AINT 2-3' |
element-specific |
I-actin |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009). |
in-house validation |
none |
1 |
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 |
element-specific |
CS-cry1A_105-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). |
in-house validation |
none |
1 |
QL-ELE-Cry1F-F2/Cry1Fr-n2 |
element-specific |
CS-cry1F_tr-BACTA |
Qualitative real-time PCR (TaqMan) method for detection of the cry1F gene (Scholtens et al., 2013, with minor modifications in forward primer and probe sequence) |
in-house validation |
none |
1 |
QL-ELE-cry2Ab2 - F/cry2Ab2 - R |
element-specific |
CS-cry2Ab2-BACTK |
Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011). |
in-house validation |
none |
1 |
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2 |
element-specific |
CS-cry3Bb1-BACUK |
Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD). |
in-house validation |
none |
1 |
QL-ELE-epsps 1-5'/epsps 3-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013). |
in-house validation |
none |
1 |
QL-ELE-epsps2 1-5'/epsps2 1-3' |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) |
in-house validation |
none |
1 |
QL-ELE-Patf-n2/Patr-n2 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006) |
in-house validation |
none |
1 |
QL-ELE-PFMV 1-5'/PFMV 1-3' |
element-specific |
P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009) |
in-house validation |
none |
1 |
QL-ELE-P-FMV-F/P-FMV-R |
element-specific |
P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-P-Rice actin-F/P-Rice actin-R |
element-specific |
P-act1-ORYSA |
Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 promoter (P-ractI) (Debode et al. 2013). |
in-house validation |
none |
1 |
QL-ELE-P-Ubi-F/P-Ubi-R |
element-specific |
P-ubi1-MAIZE |
Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-T-35S-F/T-35S-R |
element-specific |
T-Cauliflower mosaic virus |
Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013) |
in-house validation |
none |
1 |
QL-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-004 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-005 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-006 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31) |
ring trial validation |
national standard |
1 |
QL-ELE-00-007 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998) |
ring trial validation |
national standard |
1 |
QL-ELE-00-009 |
element-specific |
T-nos-RHIRD |
Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-010 |
element-specific |
E-FMV, P-34S FMV |
Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-011 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-012 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-013 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).
Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1) |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-015 |
element-specific |
E-FMV, P-34S FMV |
Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5). |
ring trial validation |
ISO standard |
1 |
QL-ELE-00-017 |
element-specific |
P-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-018 |
element-specific |
T-nos-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-019 |
element-specific |
CS-CP4epsps-RHIRD |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-021 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-ELE-00-023 |
element-specific |
T-Cauliflower mosaic virus |
Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017). |
ring trial validation |
EU reference method |
1 |
QL-ELE-00-025 |
element-specific |
CS-pat-STRVR |
Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016. |
ring trial validation |
EU reference method |
1 |
QL-TAX-ZM-002 |
taxon-specific |
Zea mays |
Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014) |
ring trial validation |
unknown |
1 |
QL-TAX-ZM-003 |
taxon-specific |
Zea mays |
Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569) |
ring trial validation |
ISO standard |
1 |
QT-CON-00-005 |
construct-specific |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2 |
Quantitative real-time PCR (TaqMan) method for detection of maize event T25 (Shindo et al., 2002) |
ring trial validation |
ISO standard |
1 |
QT-ELE-NOS ter 2-5' /NOS ter 2-3' |
element-specific |
T-nos-RHIRD |
Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009) |
ring trial validation |
none |
1 |
QT-ELE-P35S 1-5'/P35S 1-3' |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009) |
ring trial validation |
national standard |
1 |
QT-ELE-00-001 |
element-specific |
P-Cauliflower mosaic virus |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005) |
ring trial validation |
unknown |
1 |
QT-ELE-00-002 |
element-specific |
CS-pat-STRVR |
Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020) |
ring trial validation |
unknown |
1 |
QT-ELE-00-004 |
element-specific |
E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH |
Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1) |
ring trial validation |
ISO standard |
1 |
QT-EVE-ZM-010 |
event-specific |
DAS1507 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1507 (EU-RL GMFF GMOMETHODS database as of March 17, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-ZM-012 |
event-specific |
DAS59122 |
Quantitative real-time PCR (TaqMan) method for detection of maize event DAS59122 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-ZM-016 |
event-specific |
MON88017 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON88017 (EU-RL GMFF GMOMETHODS database as of March 13, 2014) |
ring trial validation |
EU reference method |
1 |
QT-EVE-ZM-018 |
event-specific |
MON89034 |
Quantitative real-time PCR (TaqMan) method for detection of maize event MON89034 (EU-RL GMFF GMOMETHODS database as of January 13, 2014) |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-001 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene.
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
ISO standard |
1 |
QT-TAX-ZM-002 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
ISO standard |
1 |
QT-TAX-ZM-003 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-004 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2 |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-005 |
taxon-specific |
Zea mays |
Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018). |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-006 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570) |
ring trial validation |
ISO standard |
1 |
QT-TAX-ZM-008 |
taxon-specific |
Zea mays |
Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-011 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014) |
ring trial validation |
EU reference method |
1 |
QT-TAX-ZM-014 |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).
Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1 |
ring trial validation |
EU reference method |
1 |
QT-TAX-zSSIIb-3F/zSSIIb-4R |
taxon-specific |
Zea mays |
Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005) |
unknown |
unknown |
1 |