Description:
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The maize BVLA430101 is modified to contain an increased amount of inorganic phosphate in the seeds. It therefore expresses a phytase gene from Aspergillus niger which degrades phytate phosphorous into organic phosphate.
The plants were transformed via particle bombardment with a construct containing the phytase gene from A. niger (phyA), which was N-terminally fused to the transit peptide of the cysteine protease 2 from Zea mays (ccp2), under the control of the promoter and termination sequence of the legumin gene from Z. mays (leg1). This cassette was derived from the vector pSPHP3303T-Phy.
They also contain a cassette for selection of transgenic plants containing the bar gene as a selectable marker. This cassette was derived from the vector pPHP17042BAR.
The maize BVLA430101 contains one complete and intact insert of the phyA2 expression cassette, including a partial reverse sequence of the histone H2B promoter from maize (P-H2b), from the bar expression cassette which was used for screening purposes. The integrity of the bar expression cassette and its number of insertions is not described.
Sources:
Chen et al. (2008), Transgenic maize plants expressing a fungal phytase gene. Transgenic Research 17:633-543.
Su et al. (2011), A construct-specific qualitative and quantitative PCR detection method of transgenic maile BVLA430101. Eur Food Res Technol 233:117–122.
Rao et al. (2016), Development of event-specific qualitative and quantitative PCR detection methods for the transgenic maize BVLA430101. Eur Food Res Technol 242:1277-1284.
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Links regarding to this GMO:
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Scientific publication - Rao et al. (2016) https://link.springer.com/article/10.1007/s00217-015-2631-7,
Scientific publication - Chen et al. (2008) https://link.springer.com/article/10.1007/s11248-007-9138-3,
Scientific publication - Su et al. (2011) https://link.springer.com/article/10.1007/s00217-011-1501-1
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Transformation / Modification technique:
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biolistic method
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