GMO molecular characterization details

GMO detailed information

GMO:
GLA safflower
Alias:

Gamma-linolenic acid safflower
UID:
Tradename:
Companies/Institutions:
Moolec Science Limited
Species:
Carthamus tinctorius
(Safflower)
Traits:
EU authorisation:

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No results found.
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General Information

Inserts / Modification

Name:	insert_GLA safflower (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the T-DNA of plasmid pSBS4119.
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	184	1-184
2	P-ole1-ARATH	Arabidopsis thaliana	1109	185-1293	Promoter of the oleosin gene from Arabidopsis thaliana. Plant et al. (1994). Plant Mol Biol 25:193-205.
3	CS-delta6D-SAPDI	Saprolegnia diclina	1363	1296-2658	Gene encoding the delta-6-desaturase enzyme from Saprolegnia diclina. Dar, A. et al., (2017). Front Plant Sci., 8, 1789. doi: 10.3389/fpls.2017.01789	Altered fatty acids and oils	cDNA sequence
4	T-ole1-ARATH	Arabidopsis thaliana	481	2663-3143	3' UTR sequence from Arabidopsis thaliana oleo1 gene.3' UTR sequence from Arabidopsis thaliana oleo1 gene.
5	P-ubi4-PETCR	Petroselinum crispum	999	3150-4148	Promoter of ubiquitin. Kawalleck, P. et al. (1993). Plant Molecular Biology 21:673-684.
6	CS-pat-STRVR	Streptomyces viridochromogenes	553	4149-4701	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37.	Glufosinate tolerance
7	T-ubi4-PETCR	Petroselinum crispum	491	4702-5192	3' UTR sequence from Petroselinum crispum ubi4 gene.
8	V-LB-RHIRD	Agrobacterium tumefaciens	312	5199-5510

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No
	USDA-APHIS	Yes	998	Ubiquitin promoter from Petroselinum crispum, part of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	1362	Delta-6-desaturase of Saprolegnia diclina, part of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	552	Phosphinothricin N-acetylfransferase (pat) from Streptomyces viridochromogenes, part of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	490	Ubiquitin terminator from Petroselinum crispum, part of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	311	Left border region of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	184	Right border region of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	1108	Oleosin promoter from Arabidopsis thaliana, part of the T-DNA of pSBS4119.
	USDA-APHIS	Yes	480	Oleosin terminator from Arabidopsis thaliana, part of the T-DNA of pSBS4119.

Detection Methods

Authorisation

Literature reference