Description:
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MT748 is a genetically modified strain of Saccharomyces cerevisiae used for the large-scale production of a precursor of an analogue of human insulin.
MT748 was developed through transformation of S. cerevisiae MT663 with the expression plasmid pMT742. This plasmid contains a synthetic gene encoding a human insulin precursor and the triose phosphate isomerase (tpi) gene. This gene, inactivated in the recipient strain and restored in the MT748 due to its presence on the plasmid, serves as selection marker.
The plasmid also contains the beta lactamase gene for ampicillin resistance as a marker gene. However, that gene is not expressed in yeast so it has no phenotypic consequence.
The inserted sequences are only present in the plasmid, there is no integration in the chromosomal DNA; the entire plasmid is present in MT748.
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Links regarding to this GMO:
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EFSA application document http://registerofquestions.efsa.europa.eu/roqFrontend/questionDocumentsLoader?question=EFSA-Q-2007-156b
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Transformation / Modification technique:
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