Description:
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GE-lepr tiger pufferfish were generated through microinjection of fertilized eggs with a single sgRNA targeting a leptin receptor gene (lepr) and a Cas9 encoding RNA. The leptin receptor gene, which is expressed in the hypothalamus of the brain is a suppressor of appetite.
A 4 bp deletion in the targeted lepr gene results in fish with increased growth rate. GE-lepr tiger pufferfish grow 1.9 times heavier than conventional tiger puffers and have an increased edible meat portion.
Putative off-targets in the genome of GE-lepr tiger pufferfish were identified. Whole genome analysis, PCR and nucleotide sequence analysis confirmed that none of the sequences had off-target mutations. The application documents indicate that the mutation was inherited through several generations and that the trait is genetically stable.
This event was first approved for commercialization in Japan in 2021.
Source: Ministry of Health, Labour and Welfare (MHLW, Japan) - Public notification document (translation of the document using DeepL)
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Links regarding to this GMO:
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MHLW document (Japanese document) https://www.mhlw.go.jp/content/11120000/000849318.pdf ,
MHLW (Japanese) https://www.mhlw.go.jp/stf/seisakunitsuite/bunya/kenkou_iryou/shokuhin/bio/genomed/newpage_00010.html ,
ISAAA - Crop Biotech Update https://www.isaaa.org/kc/cropbiotechupdate/article/default.asp?ID=19197
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Transformation / Modification technique:
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CRISPR/Cas
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