Description:
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GE-BBL Tobacco has been produced with custom-designed meganuclease. The megnuclease target is a small gene family referred to as berberine bridge enzyme-like (BBL).
The tobacco genome possesses six BBL genes and RNAi-mediated suppression of this gene
family has proven to be effective in conferring a low nicotine phenotype. Exposure of the tobacco cells to the custom-designed meganuclease targeting one or more members of the BBL family led to the production of genome-edited tobacco plants carrying small deletions and/or insertions
at the targeted loci.
The observed deletions ranged in size from 1 to 106 bp, and insertions of a maximum size of 47 bp were recovered. All observed insertion events
represented short duplications of a nearby portion of the gene being targeted by the
meganuclease.
Primary transformants were self-pollinated and transgene-free segregants with mutated BBL genes were obtained.
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Links regarding to this GMO:
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North Carolina State University USDA-APHIS inquiry https://www.aphis.usda.gov/biotechnology/downloads/reg_loi/17-126-01_air_inquiry.pdf
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Transformation / Modification technique:
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Meganuclease
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