GMO molecular characterization details

GMO detailed information

GMO:
Golden Rice 2
Alias:

GR2, GR2E
UID:
IR-ØØGR2E-5
Tradename:
Companies/Institutions:
Syngenta
Species:
Oryza sativa
(rice)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert_Golden Rice 2
Comment:
Map:

Genetic Elements:

Reference: others

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	25	1-25
2	V-MCS-SYNTH	Synthetic	194	26-219
3	P-GluA2-ORYSA	Oryza sativa	828	220-1047	Endosperm-specific glutelin promoter from rice. Takaiwa et al. (1987). FEBS Letters, 22, 43-47
4	V-MCS-SYNTH	Synthetic	39	1048-1086
5	TP-rbcS-PEA	Pisum sativum		-
6	CS-crtI-PANAN	Pantoea ananatis	1479	1258-2736	carotene desaturase I. Misawa et al. (1990). JB 172(12), 6704-6712		E. uredovora crtI functionally fused to the pea RUBISCO chloroplast transit peptide (SSUcrtI)
7	V-MCS-SYNTH	Synthetic	20	2737-2756
8	T-nos-RHIRD	Agrobacterium tumefaciens	253	2757-3009	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
9	V-intervening-SYNTH	Synthetic	16	3010-3025
10	P-GluA2-ORYSA	Oryza sativa	828	3026-3853	Endosperm-specific glutelin promoter from rice. Takaiwa et al. (1987). FEBS Letters, 22, 43-47
11	V-MCS-SYNTH	Synthetic	39	3854-3892
12	CS-psy-MAIZE	Zea mays	1233	3893-5125	Phytoene synthase gene from Zea mays. Buckner, B .et al (1996). Genetics, 143,479-488.
13	V-MCS-SYNTH	Synthetic	20	5126-5145
14	T-nos-RHIRD	Agrobacterium tumefaciens	253	5146-5398	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
15	V-MCS-SYNTH	Synthetic	25	5399-5423
16	P-ubi1-MAIZE	Zea mays	1993	5424-7416	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
17	V-MCS-SYNTH	Synthetic	12	7417-7428
18	CS-pmi-ECOLX	Escherichia coli	1176	7429-8604	Phosphomannose isomerase. Reed et al (2001). In Vitro Cell Dev Biol Plant, 37, 127-132	Mannose metabolism
19	V-MCS-SYNTH	Synthetic	60	8605-8664
20	T-nos-RHIRD	Agrobacterium tumefaciens	253	8665-8917	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
21	V-MCS-SYNTH	Synthetic	88	8918-9005
22	V-LB-RHIRD	Agrobacterium tumefaciens	25	9006-9030

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

others No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	others	No

Detection Methods

16 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	2
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-OS-002	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice Phospholipase D alpha 2 gene (Mazzara et al., 2006).	in-house validation	unknown	1
QL-TAX-OS-003	taxon-specific	Oryza sativa	Qualitative real-time PCR (TaqMan) method for detection of rice root-specific GOS9 gene (Hernandez et al., 2005)	ring trial validation	national standard	1
QL-TAX-OS-006	taxon-specific	Oryza sativa	Qualitative conventional PCR method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-EVE-OS-001	event-specific	Golden Rice 2	Quantitative real-time PCR (TaqMan) method for detection of rice event Golden Rice 2 (EU-RL GMFF GMOMETHODS database as of April 19, 2016)	ring trial validation	EU reference method	3
QT-TAX-OS-002	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016)	ring trial validation	EU reference method	1
QT-TAX-OS-003	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of rice sucrose-phosphate synthase gene (Jiang et al., 2009)	ring trial validation	unknown	1
QT-TAX-OS-017	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for the detection of rice phospholipase D alpha 2 (PLD) gene (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-TAX-SPS primer f/SPS primer r	taxon-specific	Oryza sativa	Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1)	ring trial validation	ISO standard	1

Description:	The event Golden Rice 2 was created by Agrobacterium mediated transformation with the vector pSYN12424 to produce beta (β)-carotene (a form of provitamin A) in the endosperm of the rice grain. It contains the phytoene synthase (psy) gene from Zea mays, the carotene desaturase I (crtI) gene from Pantoea ananatis, and the phosphomannose isomerase (pmi) gene from Escherichia coli as a selectable marker. Molecular characterization of the introduced DNA within Golden Rice 2 confirmed the presence at a single insertion site of one copy of the transfer-DNA (T-DNA) region derived from plasmid pSYN12424 that was stably inherited over multiple generations as a single genetic locus according to Mendelian rules of inheritance. In addition, nucleotide sequencing of the entire inserted DNA, including portions of the 5’ and 3’ flanking rice genomic sequence, confirmed that the T-DNA was inserted without modifications, deletions, or rearrangements, except for small truncations at the 5’ and 3’ termini of 23 bp and 11 bp, respectively. Sources: Food Standards Austalia New Zealand (FSANZ) Application A1138 Paine et al. (2005) Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Nature Biotechnology, 23(4), 482-487
Links regarding to this GMO:	FSANZ http://www.foodstandards.gov.au/code/applications/Documents/A1138%20SD1.pdf
Transformation / Modification technique:	Agrobacterium-induced

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

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Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference