GMO molecular characterization details

GMO detailed information

GMO:
MIR162
Alias:
UID:
SYN-IR162-4
Tradename:

Agrisure Viptera
Companies/Institutions:
Syngenta
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_MIR162
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait
1	V-Flanking Ti-nos-RHIRD	Agrobacterium tumefaciens	-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the nopaline type; sequence located in the promoter of nopaline synthase.
2	P-ubi1-MAIZE	Zea mays	-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
3	CS-vip3Aa20-BACTU	Bacillus thuringiensis	-	vegetative insecticidal protein, nearly identical in amino acid sequence to that encoded by the native vip3Aa1 gene from B. thuringiensis, with the exception of two amino acids (Lys284 changed to Glu; Met129 changed to Ile). GenBank: DQ539888.1.
4	I-9_pepc-MAIZE	Zea mays	-
5	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
6	P-ubi1-MAIZE	Zea mays	-	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
7	CS-pmi-ECOLX	Escherichia coli	-	Phosphomannose isomerase. Reed et al (2001). In Vitro Cell Dev Biol Plant, 37, 127-132	Mannose metabolism
8	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
HI203339	NCBI - GenBank	Yes	10579	Sequence 49 from Patent EP2032700, sequence of event MIR162 (including amplicon for method QT-EVE-ZM-022)
RIKILT_MIR162_event	Wageningen Food Safety Research (WFSR)	Yes	92	Amplicon sequence of MIR162 for event-specific method QT-EVE-ZM-022 without probe
RIKILT_Vip3a_element_MIR162	Wageningen Food Safety Research (WFSR)	Yes	97	Amplicon sequence of Vip3a for element-specific method QL-ELE-Vip3A-F/Vip3A-R without probe

Detection Methods

23 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-ELE-Vip3A-F/Vip3A-R	element-specific	CS-vip3Aa20-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the vip3A gene (Liang et al., 2013).	in-house validation	none	3
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	3
QT-EVE-ZM-022	event-specific	MIR162	Quantitative real-time PCR (TaqMan) method for detection of maize event MIR162 (EU-RL GMFF GMOMETHODS database as of January 10, 2014)	ring trial validation	EU reference method	3
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-008	taxon-specific	Zea mays	Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-zSSIIb-3F/zSSIIb-4R	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)	unknown	unknown	1

Catalogue number	Source	Value	Type	Certified	Available
AOCS 0917-A	AOCS	<0.1% (m/m)	dried powder	yes	yes
AOCS 0919-A	AOCS	<0.04 % (m/m)	dried powder	yes	yes
AOCS 1208-A	AOCS	>99.88 % (m/m)	dried powder	yes	no
AOCS 1208-A3	AOCS	>99.3 % (m/m)	dried powder	yes	yes
5211516301	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Commission decision number	Expiry date
1	EFSA-GMO-RX-025	Zea mays (maize, corn)	MIR162	food feed cultivation other	Syngenta Crop Protection		12.10.2033
2	EFSA-GMO-DE-2010-82	Zea mays (maize, corn)	MIR162	food feed cultivation other	Syngenta Seeds SAS, France		17.10.2022

Citation	Publication year	Publishing authority	Publication type	Content types
Commission Implementing Decision (EU) 2023/2143 of 13 October 2023 renewing the authorization for placing on the market of products containing, consisting of or produced from genetically modified maize MIR162 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council and amending Commission Implementing Decisions (EU) 2016/1685, (EU) 2019/1305 and (EU) 2019/2087 as regards the reference material	2023	EU	EU Commission Decision	decision document
Assessment of genetically modified maize MIR162 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-025)	2022	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for renewal of the authorisation of MIR162 maize import in the European Union under Articles 11 and 23 of Regulation (EC) No 1829/2003, EFSA-GMO-RX-025, Part III - Summary	2021	EFSA	application summary	application summary
COMMISSION IMPLEMENTING DECISION of 18 October 2012 authorising the placing on the market of products containing, consisting of, or produced from genetically modified maize MIR162 (SYN-IR162-4) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2012) 7198) (2012/651/EU)	2012	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-DE-2010-82) for the placing on the market of the genetically modified maize MIR162 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta	2012	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on application (EFSA-GMO-DE-2010-82) for the placing on the market of insect-resistant genetically modified maize MIR162 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta. EFSA Panel on Genetically Modified Organisms (GMO)	2012	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for authorization of MIR162 maize in the European Union under Regulation (EC) No 1829/2003 PART II: SUMMARY	2010	EFSA	application summary	application summary

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference