GMO molecular characterization details

GMO detailed information

GMO:
123.2.2
Alias:

former alias: FLO-040619-7, Moonshade, 40619
UID:
FLO-Ø4Ø619-8
Tradename:
Companies/Institutions:
Florigene, Suntory
Species:
Dianthus caryophyllus
(carnation)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

Inserts / Modification

Name:	insert_123.2.2
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-35S-CaMV	Cauliflower mosaic virus		-
2	CS-SuRB-TOBAC	Nicotiana tabacum		-	Acetohydroxy acid synthase gene (SuRB). Mazur et al. (1987). Plant Physiol 85, 1110-1117	Sulfonylurea tolerance
3	T-SuRB-TOBAC	Nicotiana tabacum		-	3' untranslated region (3' UTR) of the Nicotiana tabacum Acetohydroxy acid synthase gene. Mazur et al. (1987) Plant Physiol, 85(4): 1110-1117
4	P-chs-ANTMA	Antirrhinum majus		-	Promoter of the chalcone synthase gene. Sommer et al. (1988). Mol Gen Genet, 211, 49-55
5	CS-F3_5_H-PETHY	Petunia hybrida		-	Flavonoid 3',5'-hydroxylase (F3'5'H) gene from Petunia hybrida; is a cytochrome P450 type mono-oxygenase. Tanaka and Brugliera (2013). Philos Trans R Soc Lond B Biol Sci, 368(1612):20120432 doi: 10.1098/rstb.2012.0432. Patent WO2004020637A9	Colour modification
6	T-D8-PETHY	Petunia hybrida		-	3' untranslated region (3' UTR) of the putative phospholipid transfer protein D8 from Petunia hybrida.
7	P-mac1-SYNTH	Synthetic		-
8	CS-dfr-PETHY	Petunia hybrida		-	Dihydroflavonol-4-reductase gene. Beld et al. (1989). Plant Mol Biol, 13, 491-502	Colour modification
9	T-mas-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the mannopine synthase (mas) gene. Velten et al. (1984) EMBO J. 3:2723-30
	V-beta-gal-ECOLX	Escherichia coli		-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Transformation Vectors

Name:	pCGP1470
Comment:
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens		-
2	P-35S-CaMV	Cauliflower mosaic virus		-
3	L-cab-PETHY	Petunia hybrida		-
4	CS-SuRB-TOBAC	Nicotiana tabacum		-	Acetohydroxy acid synthase gene (SuRB). Mazur et al. (1987). Plant Physiol 85, 1110-1117	Sulfonylurea tolerance
5	T-SuRB-TOBAC	Nicotiana tabacum		-	3' untranslated region (3' UTR) of the Nicotiana tabacum Acetohydroxy acid synthase gene. Mazur et al. (1987) Plant Physiol, 85(4): 1110-1117
6	P-chs-ANTMA	Antirrhinum majus		-	Promoter of the chalcone synthase gene. Sommer et al. (1988). Mol Gen Genet, 211, 49-55
7	CS-F3_5_H-PETHY	Petunia hybrida		-	Flavonoid 3',5'-hydroxylase (F3'5'H) gene from Petunia hybrida; is a cytochrome P450 type mono-oxygenase. Tanaka and Brugliera (2013). Philos Trans R Soc Lond B Biol Sci, 368(1612):20120432 doi: 10.1098/rstb.2012.0432. Patent WO2004020637A9	Colour modification
8	T-D8-PETHY	Petunia hybrida		-	3' untranslated region (3' UTR) of the putative phospholipid transfer protein D8 from Petunia hybrida.
9	P-mac1-SYNTH	Synthetic		-
10	CS-dfr-PETHY	Petunia hybrida		-	Dihydroflavonol-4-reductase gene. Beld et al. (1989). Plant Mol Biol, 13, 491-502	Colour modification
11	T-mas-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the mannopine synthase (mas) gene. Velten et al. (1984) EMBO J. 3:2723-30
12	V-RB-RHIRD	Agrobacterium tumefaciens		-

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	application documents	No

Detection Methods

9 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-DC-001	taxon-specific	Dianthus caryophyllus	Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016).	in-house validation	EU reference method	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1

Authorisation

Literature reference