GMO molecular characterization details

GMO detailed information

GMO:
Z6
Alias:

Event Z6-Elevate, Gen2-Z6
UID:
SPS-ØØØZ6-5
Tradename:

Innate
Companies/Institutions:
SPS International Inc (SPSII)
Species:
Solanum tuberosum
(potato)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert1_Z6
Comment:
Map:

Genetic Elements:

Reference: FSANZ

Elements:

Order	Name	Donor	Position	Definition	Comment
1	V-LB-SYNTH	Synthetic	-
2	P-Agp-SOLTU	Solanum tuberosum	-
3	CS-Asn1_RNAi_as-SOLTU	Solanum tuberosum	-		Fragment of the Asn1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
4	CS-ppo5_RNAi_as-SOLVR	Solanum verrucosum	-		3' untranslated sequence of Ppo5; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
5	R-spacer		-		Sequence from S. tuberosum var. Ranger Russet between the 1st inverted repeat; transcript forms loop in dsRNA.
6	CS-ppo5_RNAi_s-SOLVR	Solanum verrucosum	-		3' untranslated sequence of Ppo5; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
7	CS-Asn1_RNAi_s-SOLTU	Solanum tuberosum	-		Fragment of the Asn1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
8	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30
9	P-Agp-SOLTU	Solanum tuberosum	-
10	CS-phl_RNAi_as-SOLTU	Solanum tuberosum	-		Fragment of promoter for the potato Phl gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
11	CS-R1_RNAi_as-SOLTU	Solanum tuberosum	-		Fragment of promoter for the potato R1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
12	R-spacer		-		Sequence from S. tuberosum var. Ranger Russet between the 2nd inverted repeat; transcript forms loop in dsRNA.
13	CS-R1_RNAi_s-SOLTU	Solanum tuberosum	-		Fragment of promoter for the potato R1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
14	CS-phl_RNAi_s-SOLTU	Solanum tuberosum	-		Fragment of promoter for the potato Phl gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
15	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30	One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
16	V-RB-SYNTH	Synthetic	-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

FSANZ No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	FSANZ	No

Name:	insert2_Z6
Comment:
Map:

Genetic Elements:

Reference: FSANZ

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-LB-SYNTH	Synthetic	-
2	P-rpi1-SOLVN	Solanum venturii	-	Native promoter of the late blight resistance gene (Rpi-vnt1).
3	CS-rpi1-SOLVN	Solanum venturii	-	Phytophthora infestans resistance gene 1. Foster et al. (2009). MPMI, 22, 589-600	Fungus and Oomycete resistance
4	T-rpi1-SOLVN	Solanum venturii	-	3' untranslated region (3' UTR) of the phytophthora/ late blight resistance gene (rpi1-vnt1). Foster et al. (2009) MPMI 22(5):589-600
5	P-Agp-SOLTU	Solanum tuberosum	-			Convergent orientation relative to the P-gbss; drives expression of an inverted repeat containing fragments of acid invertase gene.
6	CS-Vinv_RNAi_s-SOLTU	Solanum tuberosum	-	Fragment of the vacuolar acid invertase (Vinv) isolated from Solanum tuberosum in sense orientation. Ye, J. et al. (2010). J Agric Food Chem 58(23), 12162-12167	Reduced acrylamide potential	Fragment of the acid invertase gene; generates with the corresponding antisense copy double stranded RNA that triggers the degradation of invertase transcripts.
7	R-spacer		-			Fragment of the Vinv gene from S. tuberosum var. Ranger Russet. Sequence between the inverted repeat; transcript forms loop in dsRNA.
8	CS-Vinv_RNAi_as-SOLTU	Solanum tuberosum	-	Fragment of the vacuolar acid invertase (Vinv) isolated from Solanum tuberosum in antisense orientation. Ye, J. et al. (2010). J Agric Food Chem 58(23), 12162-12167	Reduced acrylamide potential	Fragment of the acid invertase gene; generates with the corresponding sense copy double stranded RNA that triggers the degradation of invertase transcripts.
9	P-gbss-SOLTU	Solanum tuberosum	-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30		Convergent orientation relative to the P-Agp; drives expression of an inverted repeat containing fragments of acid invertase gene, especially in tuber.
10	V-RB-SYNTH	Synthetic	-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

FSANZ No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	FSANZ	No

Transformation Vectors

Name:	vector_pSIM1278
Comment:	The plasmid pSIM1278 is a binary transformation vector consisting of two parts: the vector backbone and the DNA insert. The backbone section contains well-characterized bacterial origins of replication from plasmids pVS1 and pBR322, and the nptII gene for bacterial resistance to kanamycin. An expression cassette comprising the Agrobacterium ipt gene preceded by the polyubiquitin (Ubi7) promoter (GenBank accession no. U26831.1) and followed by the Ubi3 terminator was introduced as a 2.6-kb SacII fragment into the vector backbone (Garbarino and Belknap 1994). Only the DNA insert (region between left border and right border) is shown. Source: Described in USDA-APHIS petition 13-022-01p (p29-31) and petition 15-140-01p (p21-22). Entered according to petition 15-140-01p (position of the genetic elements amended; more intervening sequences described, same order of the elements). https://www.aphis.usda.gov/brs/aphisdocs/15_14001p.pdf
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Comment
1	V-LB-SYNTH	Synthetic	25	1-25
2	V-MCS-SYNTH	Synthetic	168	26-193		Includes KpnI restriction site.
3	P-Agp-SOLTU	Solanum tuberosum	2260	194-2453		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
4	CS-Asn1_RNAi_as-SOLTU	Solanum tuberosum	405	2454-2858		Fragment of the Asn1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
5	CS-ppo5_RNAi_as-SOLVR	Solanum verrucosum	144	2859-3002		3' untranslated sequence of Ppo5; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
6	V-intervening-SOLTU	Solanum tuberosum	163	3003-3165		Includes XbaI restriction site.
7	CS-ppo5_RNAi_s-SOLVR	Solanum verrucosum	144	3166-3309		3' untranslated sequence of Ppo5; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Ppo5 transcripts to block black spot bruise development.
8	CS-Asn1_RNAi_s-SOLTU	Solanum tuberosum	406	3310-3715		Fragment of the Asn1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degradation of Asn1 transcripts to impair asparagine formation.
9	V-intervening-SOLTU	Solanum tuberosum	6	3716-3721		EcoRI restriction site.
10	P-gbss-SOLTU	Solanum tuberosum	686	3722-4407	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30	One of the two convergent promoters that drives expression of an inverted repeat containing fragments of Asn1 and Ppo5, especially in tubers.
11	V-intervening-SOLTU	Solanum tuberosum	16	4408-4423		Includes SpeI and KpnI restritcion sites.
12	P-Agp-SOLTU	Solanum tuberosum	2260	4424-6683		One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
13	CS-phl_RNAi_as-SOLTU	Solanum tuberosum	509	6684-7192		Fragment of promoter for the potato Phl gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
14	CS-R1_RNAi_as-SOLTU	Solanum tuberosum	532	7193-7724		Fragment of promoter for the potato R1 gene; generates with the corresponding copy in sense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
15	V-intervening-SOLTU	Solanum tuberosum	264	7725-7988		Includes PstI restriction site.
16	CS-R1_RNAi_s-SOLTU	Solanum tuberosum	532	7989-8520		Fragment of promoter for the potato R1 gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of R1 transcripts to limit the formation of reduicing sugars through starch degradation.
17	CS-phl_RNAi_s-SOLTU	Solanum tuberosum	509	8521-9029		Fragment of promoter for the potato Phl gene; generates with the corresponding copy in antisense orientation double stranded RNA that triggers the degredation of PhL transcripts to limit the formation of reducing sugars through starch degradation.
18	P-gbss-SOLTU	Solanum tuberosum	924	9030-9953	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30	One of the two convergent promoters that drives expression of an inverted repeat containing fragments of the promoters of PhL and R1, especially in tubers.
19	V-MCS-SYNTH	Synthetic	170	9954-10123		Includes SacI restriction site.
20	V-RB-SYNTH	Synthetic	25	10124-10148

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No	10148

Name:	vector_pSIM1678
Comment:	The pSIM1678 insert consists of the rpi1 expression cassette and a silencing cassette for the plant vacuolar invertase gene (Vinv) also called acid invertase (AI). The rpi1 gene cassette consists of the rpi1 protein coding region from _Solanum venturii_ regulated by its native promoter and terminator sequences, whereas the silencing cassette consists of an inverted repeat of sequence from the potato AI gene flanked by opposing plant promoters, P-gbss and P-Agp.
Map:	show

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-SYNTH	Synthetic	25	1-25
2			168	26-193			Includes a KpnI restriction site.
3	P-rpi1-SOLVN	Solanum venturii	709	194-902	Native promoter of the late blight resistance gene (Rpi-vnt1).
4	CS-rpi1-SOLVN	Solanum venturii	2676	903-3578	Phytophthora infestans resistance gene 1. Foster et al. (2009). MPMI, 22, 589-600	Fungus and Oomycete resistance
5	T-rpi1-SOLVN	Solanum venturii	925	3579-4503	3' untranslated region (3' UTR) of the phytophthora/ late blight resistance gene (rpi1-vnt1). Foster et al. (2009) MPMI 22(5):589-600
6	V-intervening-SOLTU	Solanum tuberosum	6	4504-4509			Apa1 restriction site.
7	P-Agp-SOLTU	Solanum tuberosum	2261	4510-6770			Convergent orientation relative to the P-gbss; drives expression of an inverted repeat containing fragments of acid invertase gene.
8	V-intervening-SOLTU	Solanum tuberosum	6	6771-6776			BamHI restriction site.
9	CS-Vinv_RNAi_s-SOLTU	Solanum tuberosum	498	6777-7274	Fragment of the vacuolar acid invertase (Vinv) isolated from Solanum tuberosum in sense orientation. Ye, J. et al. (2010). J Agric Food Chem 58(23), 12162-12167	Reduced acrylamide potential	Fragment of the AI gene; generates with the corresponding antisense fragment a double stranded RNA that triggers the degradation of invertase transcripts.
10	R-spacer		181	7275-7455			Vinv gene fragment from S. tuberosum var. Ranger Russet. Sequence between the inverted repeat; transcript forms loop in dsRNA.
11	V-intervening-SOLTU	Solanum tuberosum	6	7456-7461			EcoRI restriction site.
12	CS-Vinv_RNAi_as-SOLTU	Solanum tuberosum	504	7462-7965	Fragment of the vacuolar acid invertase (Vinv) isolated from Solanum tuberosum in antisense orientation. Ye, J. et al. (2010). J Agric Food Chem 58(23), 12162-12167	Reduced acrylamide potential	Fragment of the AI gene; generates with the corresponding sense fragment a double stranded RNA that triggers the degradation of invertase transcripts.
13	V-intervening-SOLTU	Solanum tuberosum	6	7966-7971			Spe1 restriction site.
14	P-gbss-SOLTU	Solanum tuberosum	924	7972-8895	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30		Convergent orientation relative to the P-Agp; drives expression of an inverted repeat containing fragments of acid invertase gene.
15	V-MCS-SYNTH	Synthetic	170	8896-9065			Includes a SacI restriction site.
16	V-RB-SYNTH	Synthetic	25	9066-9090

Related DNA Sequences

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No	9090

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Related DNA Sequences

Related DNA Sequences

Detection Methods

Reference Materials

Authorisation

Literature reference