GMO molecular characterization details

GMO detailed information

GMO:
F3'5'H GM petunia
Alias:

GM petunia containing a flavonoid 3', 5' hydroxylase expression cassette
UID:
Tradename:
Companies/Institutions:
Species:
Petunia hybrida
(petunia)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

Inserts / Modification

Name:	insert_F3'5'H GM petunia
Comment:	Molecular analyses suggest the following order of elements for cassettes present in the construct. The order of cassettes is not clear. Moreover, it is possible that further genetic elements are present in one or more of the GM petunia varieties. Further source: Shimada et al. (1999) Expression of chimeric P450 genes encoding flavonoid-3′,5′-hydroxylase in transgenic tobacco and petunia plants. FEBS Lett 461:241–245
Map:

Genetic Elements:

Reference: others

Elements:

Order	Name	Donor	Position	Definition	Trait
1	P-nos-RHIRD	Agrobacterium tumefaciens	-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
2	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
3	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
4	P-Cauliflower mosaic virus		-
5	CS-F3_5_H-PETHY	Petunia hybrida	-	Flavonoid 3',5'-hydroxylase (F3'5'H) gene from Petunia hybrida; is a cytochrome P450 type mono-oxygenase. Tanaka and Brugliera (2013). Philos Trans R Soc Lond B Biol Sci, 368(1612):20120432 doi: 10.1098/rstb.2012.0432. Patent WO2004020637A9	Colour modification
6	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807

Related DNA Sequences:

Reference	Sequence	Size (bp)	Annotation
others	No
others	Yes	974	1-768 bp correspond to F3'5'H segment from Petunia hybrida 769-974 bp correspond to T-nos segment from Agrobacterium tumefaciens
others	Yes	1015	1-278 bp correspond to P-35S segment from Cauliflower mosaic virus 279-1015 bp correspond to F3'5'H segment from Petunia hybrida

Detection Methods

21 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-014	construct-specific	1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1

Description:	F3'5'H GM petunia is not a specific event but a group of petunia varieties that have been modified for altered flower color through the insertion of a flavonoid 3', 5' hydroxylase expression cassette. The petunia have been tested positive for the listed genetic elements.
Links regarding to this GMO:	BCH http://bch.cbd.int/database/record.shtml?documentid=112258
Transformation / Modification technique:

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

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Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

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Literature reference