Description:
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E12 was developed through Agrobacterium-mediated transformation of the potato variety Russet Burbank with plasmid vector pSIM1278. The intended consequence of the genetic modification in E12 is to silence genes that lead to acrylamide formation in cooked potatoes, and genes that cause browning in damaged potatoes.
E12 was confirmed to be free of Agrobacterium-derived vector backbone DNA.
Southern hybridizations showed that E12 contain a single DNA insert comprising all sequences needed for expression of both the Asn1/Ppo5 gene silencing cassette and R1/PhL promoter silencing cassette. Molecular analysis and an enzymatic assay indicated that the DNA insert was stable through at least the second generation.
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Links regarding to this GMO:
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bch http://bch.cbd.int/database/record.shtml?documentid=106428,
USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/13_02201p.pdf
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Transformation / Modification technique:
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Agrobacterium-induced
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