GMO molecular characterization details

GMO detailed information

GMO:
GHB614 x LLCotton25 x MON15985
Alias:
UID:
BCS-GHØØ2-5 x ACS-GHØØ1-3 x MON-15985-7
Tradename:
Companies/Institutions:
Bayer CropScience, Bayer Crop Science (formerly Monsanto Company), Aventis CropScience
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

no Reference materials could be found

Detection Methods

37 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-015	construct-specific	Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC)	in-house validation	none	1
QL-CON-35S-F/nptII-R	construct-specific	23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010).	in-house validation	none	1
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2	element-specific	CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-cry2Ab2 - F/cry2Ab2 - R	element-specific	CS-cry2Ab2-BACTK	Qualitative real-time PCR (TaqMan) method for detection of the cry2Ab2 gene (Dinon et al. 2011).	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QT-CON-00-008	construct-specific	DAS44406, FG72, GA21, GHB614, GHB811	Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002)	ring trial validation	ISO standard	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1
QT-EVE-GH-002	event-specific	LLCotton25	Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-EVE-GH-005	event-specific	MON15985	Quantitative real-time PCR (TaqMan) method for the detection of cotton event MON15985 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-EVE-GH-006	event-specific	GHB614	Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	1
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1

Description:	For Reference Materials, please consult the materials mentioned under the separate GMO events.
Links regarding to this GMO:
Transformation / Modification technique:	conventional crossing

Citation	Publication year	Publishing authority	Publication type	Content types
Commission Implementing Decision (EU) 2022/560 of 31 March 2022 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified cotton GHB614 (BCS-GHØØ2-5) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2022) 1891)	2022	EU	EU Commission Decision	decision document
Assessment of genetically modified cotton GHB614 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA–GMO–RX–018)	2021	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Request for Renewal of the Authorisation of the genetically modified herbicide tolerant cotton GHB614 for food and feed uses, and import and processing, in accordance with articles 11 and 23 of Regulation (EC) No 1829/2003, EFSA-GMO-RX-018, Part III - Summary	2020	EFSA	application summary	application summary
COMMISSION IMPLEMENTING DECISION (EU) 2019/2082 of 28 November 2019 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified cotton LLCotton25 (ACS-GHØØ1-3) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2019) 7481)	2019	EU	EU Commission Decision	decision document
Commission Implementing decision (EU) 2019/1302 of 26 July 2019 authorising the placing on the market of products containing, consisting of or produced from genetically modified cotton GHB614 × LLCotton25 × MON 15985 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2019) 5501)	2019	EU	EU Commission Decision	decision document
Assessment of genetically modified LLCotton25 for renewal of authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-010)	2018	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority on genetically modified cotton GHB614 × LLCotton25 × MON 15985 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA-GMO-NL-2011-94)	2018	EFSA	EFSA overall opinion	risk assessment document
Overall opinion of the European Food Safety Authority on genetically modified LLCotton25 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-010)	2018	EFSA	EFSA overall opinion	risk assessment document
Assessment of genetically modified cotton GHB614 x LLCotton25 x MON 15985 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA-GMO-NL-2011-94)	2018	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Request for Renewal of the Authorisation of the genetically modified herbicide tolerant cotton LLCotton25 for food and feed uses, import and processing, in accordance with Articles 11 and 23 of Regulation (EC) No 1829/2003	2017	EFSA	application summary	application summary
COMMISSION IMPLEMENTING DECISION (EU) 2015/685 of 24 April 2015 authorising the placing on the market of genetically modified cotton MON 15985 (MON-15985-7) and renewing the authorisation for existing genetically modified cotton MON 15985 (MON-15985-7) products pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2015) 2755)	2015	EU	EU Commission Decision	decision document
Request for Authorization in accordance with the articles 5 and 17 of Regulation (EC) 1829/2003 GM Food and GM Feed Glyphosate and glufosinate ammonium-tolerant and insect-resistant GM cotton GHB614 x LLCotton25 x MON 15985, and the subcombination LLCotton25 x MON 15985 for food and feed uses, and for import and processing - PART II - Summary	2015	EFSA	application summary	application summary
Application for authorization to place on the market MON 15985 cotton in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed Part II Summary	2014	EFSA	application summary	application summary
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-UK-2008-57) for the placing on the market of insect-resistant genetically modified cotton MON 15985 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 from Monsanto	2014	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion on applications (EFSA-GMO-UK-2008-57 and EFSA-GMO-RX-MON15985) for the placing on the market of insect-resistant genetically modified cotton MON 15985 for food and feed uses, import and processing1, and for the renewal of authorisation of existing products produced from cotton MON 159852, both under Regulation (EC) No 1829/2003 from Monsanto	2014	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
PART VII Summary of the application for the Request for Authorization of genetically modified herbicide tolerant cotton for cultivation in accordance with Regulation (EC) No 1829/2003.	2013	EFSA	application summary	application summary
COMMISSION DECISION of 17 June 2011 authorising the placing on the market of products containing, consisting of, or produced from genetically modified cotton GHB614 (BCS-GHØØ2-5) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2011) 4177) (2011/354/EU)	2011	EU	EU Commission Decision	decision document
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.	2009	Research group	peer-reviewed article	safety
Opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No. 1829/2003 on application EFSA-GMO-NL-2008-51 Application for the placing on the market of herbicide tolerant genetically modified cotton GHB614 for food and feed uses from Bayer CropScience AG. (Question No. EFSA-Q-2008-016)	2009	EFSA	EFSA overall opinion	risk assessment document
Herouet-Guicheney, C., Rouquie, D., Freyssinet, M., Currier, T., Martone, A., Zhou, J., . . . Rouan, D. (2009). Safety evaluation of the double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mEPSPS) from maize that confers tolerance to glyphosate herbicide in transgenic plants. Regulatory Toxicology and Pharmacology, 54(2), 143-153.	2009	Research group	peer-reviewed article	safety
SCIENTIFIC OPINION Application (Reference EFSA-GMO-NL-2008-51) for the placing on the market of glyphosate tolerant genetically modified cotton GHB614, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience1 Scientific Opinion of the Panel on Genetically Modified Organisms (Question No EFSA-Q-2008-016)	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Part II Summary of the application for request the authorisation Glufosinate-ammonium tolerant cotton LLCotton25 for food and feed uses, and import and processing, in accordance with articles 5 and 17 of regulation 1829/2003 GM food and GM feed, and for industrial uses	2008	EFSA	application summary	application summary
Application for authorization of MON15985 and MON15985 x MON1445 cotton in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed - Part II - Summary	2008		application summary	application summary
COMMISSION DECISION of 29 October 2008 authorising the placing on the market of products containing, consisting of, or produced from genetically modified LLCotton25 (ACS-GHØØ1-3) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document number C(2008) 6204) (2008/837/EC)	2008	EU	EU Commission Decision	decision document
PART II SUMMARY OF THE APPLICATION FOR REQUEST FOR AUTHORIZATION GLYPHOSATE-TOLERANT COTTON GHB614 FOR FOOD AND FEED USES, AND IMPORT AND PROCESSING, IN ACCORDANCE WITH ARTICLES 5 AND 17 OF REGULATION 1829/2003 GM FOOD AND GM FEED, AND FOR INDUSTRIAL USES	2008	EFSA	application summary	application summary
Application for renewal of the authorization for continued marketing of existing food additives, feed materials and feed additives produced from MON 15985 and MON 15985 × MON 1445 cotton that were notified according to Articles 8(1)(b) and 20(1)(b) of Regulation (EC)No 1829/2003 on genetically modified food and feed	2007	EFSA	application summary	application summary
Opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No. 1829/2003 on application EFSA-GMO-NL-2005-13 Application for the placing on the market of glufosinate-tolerant genetically modified LLcotton25 for food and feed uses, import and processing from Bayer CropScience (Question No. EFSA-Q-2005-047)	2007	EFSA	EFSA overall opinion	risk assessment document
Mazzara M., Grazioli E., Savini C., Van Den Eede G.; Event-Specific Method for the Quantification of Cotton Line LLCotton25" Using Real-time PCR - Validation Report and Protocol - Cotton Seeds Sampling and DNA Extraction" Online Publication (2007)	2007		EU reference method protocol	detection
Opinion of the Scientific Panel on Genetically Modified Organisms on an application (Reference EFSA-GMO-NL-2005-13) for the placing on the market of glufosinate-tolerant genetically modified LLCotton25, for food and feed uses, and import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience1 (Question No EFSA-Q-2005-047) Opinion adopted on 6 December 2006	2006	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference