GMO molecular characterization details

GMO detailed information

GMO:
DAS44406
Alias:
UID:
DAS-444Ø6-6
Tradename:

Enlist E3
Companies/Institutions:
MS Technologies, DOW AgroSciences
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

Inserts / Modification

Name:	insert_DAS44406
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	E-rb7_mar-TOBAC			-
2	T-H4-ARATH	Arabidopsis thaliana		-	sequence including the 3´ untranslated region (3' UTR) of the histone H4 gene. Chaboute et al. (1987) Plant Mol Biol. 8:179-191
3	CS-epsps-MAIZE	Zea mays		-	5-enolpyruvylshikimate-3-phosphate synthase gene, modified. Lebrun et al. (2003) US6566587 B1	Glyphosate tolerance
4	TP-rbcS-SYNTH	Synthetic		-	Optimised chloroplast transit peptide derivative of the RuBisCo small unit genes from Zea mays and Helianthus annuus
5	I-1_H3-ARATH	Arabidopsis thaliana		-
6	P-H4-ARATH	Arabidopsis thaliana		-	Promoter of the histone H4 gene. Chaboute et al. (1987). Plant Mol Biol, 8, 179-191
7	P-ubi10-ARATH	Arabidopsis thaliana		-	Promoter of the Ubiquitin-10 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906
8	CS-aad12-DELAC	Delftia acidovorans		-	Aryloxyalkanoate dioxygenase 12 gene (aad12). Wright et al. (2010) PNAS 107(47):20240-20245	2,4-D tolerance
9	T-orf23-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the ORF23 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350
10	P-CsVMV	Cassava vein mosaic virus		-	Promoter region of the Cassava vein mosaic virus. Verdaguer et al. (1996). Plant Mol Biol, 31:1129-1139.
11	CS-pat-STRVR	Streptomyces viridochromogenes		-	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37	Glufosinate tolerance
12	T-orf1-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the ORF1 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
JC006926	NCBI - GenBank	Yes	13659	Sequence 27 from Patent EP2645848, sequence of event DAS44406 (including amplicon for method QT-EVE-GM-015)
RIKILT_DAS44406_event	Wageningen Food Safety Research (WFSR)	Yes	99	Amplicon sequence of DAS44406 for event-specific method QT-EVE-GM-015 without probe

Detection Methods

19 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-CsVMV F/patsyn R	construct-specific	DAS44406, DAS68416, DAS81419	Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	3
QL-ELE-00-021	element-specific	CS-pat-STRVR	Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-025	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.	ring trial validation	EU reference method	2
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-008	construct-specific	DAS44406, FG72, GA21, GHB614, GHB811	Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002)	ring trial validation	ISO standard	3
QT-ELE-00-002	element-specific	CS-pat-STRVR	Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)	ring trial validation	unknown	2
QT-EVE-GM-015	event-specific	DAS44406	Quantitative PCR method for detection of soybean event DAS44406 (EURL GMFF GMOMETHODS database as of May 28, 2015)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference