GMO molecular characterization details

GMO detailed information

GMO:
MON863 x MON810
Alias:
UID:
MON-ØØ863-5 x MON-ØØ81Ø-6
Tradename:

YieldGard Plus
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

Detection Methods

41 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-35S-F/nptII-R	construct-specific	23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010).	in-house validation	none	1
QL-ELE-AINT 2-5'/AINT 2-3'	element-specific	I-actin	Qualitative real-time PCR (TaqMan) method for detection of rice actin 1 intron (I-ractI) (Mano et al. 2009).	in-house validation	none	1
QL-ELE-cry1A 4-5'/cry1A 4-3'	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-Cry3Bbf-n2/Cry3Bbr-n2	element-specific	CS-cry3Bb1-BACUK	Qualitative real-time PCR (TaqMan) method for detection of cry3Bb gene (Scholtens et al., 2013; GMDD).	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-020	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (SYBRGreen) method for detection of CS-cry1Ab-BACTU (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-EVE-ZM-001	event-specific	MON810	Qualitative conventional PCR method for the detection of maize event MON810 (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-004	construct-specific	DBN9936, MON80100, MON802, MON809, MON810	Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (Shindo et al., 2002)	ring trial validation	ISO standard	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-003	element-specific	CS-cry1Ab-BACTU	Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570)	ring trial validation	ISO standard	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1
QT-EVE-ZM-009	event-specific	MON863	Quantitative real-time PCR (TaqMan) method for detection of maize event MON863 (EU-RL GMFF GMOMETHODS database as of March 13, 2014)	ring trial validation	EU reference method	1
QT-EVE-ZM-020	event-specific	MON810	Quantitative real-time PCR (TaqMan) method for detection of maize event MON810 (ISO/FDIS 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-008	taxon-specific	Zea mays	Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-zSSIIb-3F/zSSIIb-4R	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)	unknown	unknown	1

Description:	For Reference Materials, please also consult the materials mentioned under the separate GMO events.
Links regarding to this GMO:
Transformation / Modification technique:	conventional crossing

Catalogue number	Source	Value	Type	Certified	Available
ERM-BF417a	JRC - CRM (ERM)	<0.1% (m/m)	dried powder	yes	yes
ERM-BF417b	JRC - CRM (ERM)	0.1% (m/m)	dried powder	yes	yes
ERM-BF417c	JRC - CRM (ERM)	0.98 % (m/m)	dried powder	yes	yes
ERM-BF417d	JRC - CRM (ERM)	9.85 % (m/m)	dried powder	yes	yes

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Commission decision number	Expiry date
1	EFSA-GMO-DE-2004-03	Zea mays (maize, corn)	MON863 x MON810	food feed cultivation other	Monsanto Europe		26.01.2016
2	EFSA-GMO-RX-MON863 x MON810 (20.1.b)	Zea mays (maize, corn)	MON863 x MON810	food feed cultivation other	Monsanto Europe		26.01.2016

Citation	Publication year	Publishing authority	Publication type	Content types
Assessment of genetically modified maize MON 810 for renewal authorisation under Regulation (EC) No 1829/2003 (dossier GMFF-2022-9450)	2024	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for renewal of the authorisations to place on the market MON 810 maize under Regulation (EC) No 1829/2003 on genetically modified food and feed (Commission Implementing Decision 2013/649/EU of 6 November 2013) and (Commission Implementing Decision 2017/1207/EU of 4 July 2017). GMFF-2022-9450 RX028, Summary	2023	EFSA	application summary	application summary
Commission implementing decision (EU) 2017/1207 of 4 July 2017 renewing the authorisation for the placing on the market of genetically modified maize MON810 (MON-ØØ81Ø-6) products pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2017) 4453)	2017	EU	EU Commission Decision	decision document
Commission Implemening Decision (EU) 2016/87 of 22 January 2016 on the withdrawal from the market of existing products derived from MON863 (MON-ØØ863-5) and repealing Decisions 2010/139/EU, 2010/140/EU, 2010/141/EU authorising the placing on the market of products containing, consisting of, or produced from genetically modified maize MON863 × MON810 × NK603 (MON-ØØ863-5 × MON-ØØ81Ø-6 × MON-Ø6Ø3-6), MON863 × MON810 (MON-ØØ863-5 × MON-ØØ81Ø-6) and MON863 × NK603 (MON-ØØ863-5 × MON-ØØ6Ø3-6) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2016) 204)	2016	EU	EU Commission Decision	decision document
Commission implementing decision of 6 November 2013 authorising the placing on the market of pollen produced from maize MON 810 (MON-ØØ81Ø-6) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2013)4743) (2013/649/EU)	2013	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Article 6 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-NL-2012-107) for the placing on the market of food produced from or containing ingredients produced from maize MON 810, according to Article 3(1)(c) of Regulation (EC) No 1829/2003 (maize MON 810 pollen as or in food) (application EFSA-GMO-NL-2012-107) from Monsanto	2012	EFSA	EFSA overall opinion	risk assessment document
Application for authorization to place on the market MON 810 pollen in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed. Part VII Summary	2012	EFSA	application summary	application summary
Scientific Opinion on an application (EFSA-GMO-NL-2012-107) for the placing on the market of maize MON 810 pollen under Regulation (EC) No 1829/2003 from Monsanto	2012	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Scientific Opinion on an application (EFSA-GMO-RX-MON863) for renewal of the authorisation for continued marketing of existing feed materials, feed additives and food additives produced from maize MON863, under Regulation (EC) No 1829/2003 from Monsanto	2010	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on the application (reference EFSA-GMO-RX-MON863) for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON863 for food and feed uses from Monsanto	2010	EFSA	EFSA overall opinion	risk assessment document
Commission Decision of 2 March 2010 authorising the placing on the market of products containing, consisting of, or produced from genetically modified maize MON863xMON810 (MON-ØØ863-5xMON-ØØ81Ø-6) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (2010/140/EU)	2010	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Article 18 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[20-1a] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[20-1a], and seeds for cultivation under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA	EFSA overall opinion	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[8-1b/20-1b] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[8-1b/20-1b], under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA	EFSA overall opinion	risk assessment document
Application for renewal of the authorisation for continued marketing of existing MON810 maize products that were authorized under Directive 90/220/EEC (Decision 98/294/EC) and subsequently notified in accordance to Article 20(1)(a) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Application for renewal of the authorisation for continued marketing of existing food and food ingredients produced from MON810 maize that were notified pursuant to Article 5 of Regulation (EC) No 258/97 and subsequently notified according to Article 8(1)(a) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.	2009	Research group	peer-reviewed article	safety
Overall opinion of the European Food Safety Authority in accordance with Article 6 of Regulation (EC) No 1829/2003 on application EFSA-GMO-RX-MON810[8-1a] for renewal of the authorisation of existing products produced from insect resistant genetically modified maize MON810[8-1a], under Regulation (EC) No 1829/2003 from Monsanto.	2009	EFSA	EFSA overall opinion	risk assessment document
Scientific Opinion - Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continued marketing of (1) existing food and food ingredients produced from genetically modified insect resistant maize MON810; (2) feed consisting of and/or containing maize MON810, including the use of seed for cultivation; and of (3) food and feed additives, and feed materials produced from maize MON810, all under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for renewal of the authorisation for continued marketing of existing food additives, feed materials and feed additives produced from MON810 maize that were notified according to Articles 8(1)(b) and 20(1)(b) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2009	EFSA	application summary	application summary
Scientific Opinion of GMO Panel: Applications (EFSA-GMO-RX-MON810) for renewal of authorisation for the continued marketing of (1) existing food and food ingredients produced from genetically modified insect resistant maize MON810; (2) feed consisting of and/or containing maize MON810, including the use of seed for cultivation; and of (3) food and feed additives, and feed materials produced from maize MON810, all under Regulation (EC) No 1829/2003 from Monsanto	2009	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Application for authorization of MON 863 × MON 810 maize in the European Union, according to Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II Summary	2008	EFSA	application summary	application summary
Application for renewal of the authorisation for continued marketing of existing feed materials, feed additives and food additives produced from MON 863 maize that were previously notified, according to Articles 8(1)(b) and 20(1)(b) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2008	EFSA	application summary	application summary
Application for renewal of the authorisation for continued marketing of existing feed materials produced from MON 863 × MON 810 maize that were previously notified, according to Article 20(1)(b) of Regulation (EC) No 1829/2003 on genetically modified food and feed. Part II - Summary	2008	EFSA	application summary	application summary
Opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application EFSA-GMO-DE-2004-03 Application for the placing on the market of insect-protected genetically modified maize MON863 x MON810 for food and feed uses from Monsanto (Question No EFSA-Q-2004-112)	2006	EFSA	EFSA overall opinion	risk assessment document
Opinion of the Scientific Panel on Genetically Modified Organisms on an application (Reference EFSA-GMO-DE-2004-03) for the placing on the market of insect-protected genetically modified maize MON 863 x MON 810, for food and feed use, under Regulation (EC) No 1829/2003 from Monsanto1 (Question No EFSA-Q-2004-112)	2005	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Commission decision of 22 April 1998 concerning the placing on the market of genetically modified maize (Zea mays L. line MON 810), pursuant to Council Directive 90/220/EEC (98/294/EC)	1998	EU	EU Commission Decision	decision document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference