GMO molecular characterization details

GMO detailed information

GMO:
FG72
Alias:

EE-GM3, FG-072
UID:
MST-FGØ72-2
Tradename:

Balance GT
Companies/Institutions:
MS Technologies, Bayer CropScience
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

Inserts / Modification

Name:	insert_FG72
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear. The cassette bearing CS-epsps-MAIZE_v2 was inserted into the vector in an antisense orientation relative to the cassette bearing the coding sequence of CS-hpp-PSEFL.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait
1	T-H4-ARATH	Arabidopsis thaliana	-	sequence including the 3´ untranslated region (3' UTR) of the histone H4 gene. Chaboute et al. (1987) Plant Mol Biol. 8:179-191
2	CS-epsps-MAIZE	Zea mays	-	5-enolpyruvylshikimate-3-phosphate synthase gene, modified. Lebrun et al. (2003) US6566587 B1	Glyphosate tolerance
3	TP-rbcS-SYNTH	Synthetic	-	Optimised chloroplast transit peptide derivative of the RuBisCo small unit genes from Zea mays and Helianthus annuus
4	I-1_H3-ARATH	Arabidopsis thaliana	-
5	P-H4-ARATH	Arabidopsis thaliana	-	Promoter of the histone H4 gene. Chaboute et al. (1987). Plant Mol Biol, 8, 179-191
6	P-H4-ARATH	Arabidopsis thaliana	-	Promoter of the histone H4 gene. Chaboute et al. (1987). Plant Mol Biol, 8, 179-191
7	L-TEV	Tobacco etch virus	-
8	TP-rbcS-SYNTH	Synthetic	-	Optimised chloroplast transit peptide derivative of the RuBisCo small unit genes from Zea mays and Helianthus annuus
9	CS-HPPD-PSEFL	Pseudomonas fluorescens	-	sequence encoding a 4-hydroxyphenylpyruvate dioxygenase variant. Patent US6245968B1
10	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
RIKILT_FG72_event	Wageningen Food Safety Research (WFSR)	Yes	70	Amplicon sequence of FG72 for event-specific method QT-EVE-GM-001 without probe

Detection Methods

20 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-008	construct-specific	DAS44406, FG72, GA21, GHB614, GHB811	Quantitative real-time PCR (TaqMan) method for detection of maize event GA21 (Shindo et al., 2002)	ring trial validation	ISO standard	3
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	3
QT-EVE-GM-001	event-specific	FG72	Quantitative real-time PCR (TaqMan) method for detection of soybean event FG72 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Catalogue number	Source	Value	Type	Certified	Available
AOCS 0610-A3	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0610-A5	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0610-A5		>99.99 % (m/m)		no	no
AOCS 0610-A6	AOCS	>99.99 % (m/m)	genomic DNA	yes	yes
AOCS 0707-A7	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0707-A8	AOCS	<0.005 % (m/m)	genomic DNA	yes	no
AOCS 0707-A9	AOCS	<0.005 % (m/m)	genomic DNA	yes	yes
5211519901	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no

Citation	Publication year	Publishing authority	Publication type	Content types
COMMISSION IMPLEMENTING DECISION (EU) 2016/1215 of 22 July 2016 authorising the placing on the market of products containing, consisting of, or produced from genetically modified soybean FG72 (MST-FGØ72-2) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2016) 4576)	2016	EU	EU Commission Decision	decision document
Scientific Opinion on an application (EFSA-GMO-BE-2011-98) for the placing on the market of herbicide-tolerant genetically modified soybean FG72 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience	2015	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-BE-2011-98) for the placing on the market of herbicide-tolerant genetically modified soybean FG72 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience	2015	EFSA	EFSA overall opinion	risk assessment document
Request for Authorization of Herbicide Tolerant Genetically Modified Soybean FG72 for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) N° 1829/2003 GM Food and GM Feed Part II - Summary	2011	EFSA	application summary	application summary
Herouet-Guicheney, C., Rouquie, D., Freyssinet, M., Currier, T., Martone, A., Zhou, J., . . . Rouan, D. (2009). Safety evaluation of the double mutant 5-enol pyruvylshikimate-3-phosphate synthase (2mEPSPS) from maize that confers tolerance to glyphosate herbicide in transgenic plants. Regulatory Toxicology and Pharmacology, 54(2), 143-153.	2009	Research group	peer-reviewed article	safety

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference