Potato line PH05-026-0048 was developed through Agrobacterium-mediated transformation using plasmid vector VCPMA16. The VCPMA16 T-DNA contains:

• The acetohydroxyacid synthase (ahas) gene from Arabidopsis thaliana and has a point mutation corresponding to S653N in the expressed AHAS protein. The point mutation results in expressed protein, which confers resistance to herbicides from the Imidazolinone family used for selection during transformation.
• Two resistance genes, rpi1 and rpi2 from the wild potato Solanum bulbocastanum, which confer increased resistance to Phytophthora infestans.

PCR analyses confirmed the presence of the rpi1 and rpi2 genes as well as the ahas gene in the transgenic line PH05-026-0048.