GMO molecular characterization details

GMO detailed information

GMO:
Ms11
Alias:
UID:
BCS-BNØ12-7
Tradename:
Company:
Bayer CropScience
Developer:

Bayer CropScience
Species:
Brassica napus
(canola, oilseed rape, rapeseed)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_Ms11
Comment:
Map:

Genetic Elements:

Reference: USDA-APHIS

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	25	1-25
2	V-MCS-SYNTH	Synthetic	72	26-97
3	T-tr7-RHIRD	Agrobacterium tumefaciens	212	98-309	3' untranslated region (3' UTR) of the Agrobacterium transcript 7 T-DNA gene. Dhaese et al. (1983) EMBO Journal 2(3):419-426		Counter clockwise
4	V-MCS-SYNTH	Synthetic	22	310-331
5	CS-bar-STRHY	Streptomyces hygroscopicus	552	332-883	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523	Glufosinate tolerance	Counter clockwise
6	P-rbcS-ARATH	Arabidopsis thaliana	1730	884-2613	Promoter of the ribulose- 1,5-bisphosphate carboxylase (Rubisco) small subunit gene. Almeida et al. (1989) Mol Gen Genet, 218(1):78-86		Counter clockwise
7	V-MCS-SYNTH	Synthetic	45	2614-2658
8	T-nos-RHIRD	Agrobacterium tumefaciens	261	2659-2919	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		Counter clockwise
9	V-MCS-SYNTH	Synthetic	16	2920-2935
10	T-barnase-BACAM	Bacillus amyloliquefaciens	98	2936-3033	3' untranslated region (3' UTR) of the extracellular ribonuclease (barnase) gene. An inverted repeat at the 3' end of the gene may act as a transcription terminator. Paddon and Hartley (1986) Gene 40:231-239		Counter clockwise
11	CS-barnase-BACAM	Bacillus amyloliquefaciens	336	3034-3369	Ribonuclease gene barnase. Mariani et al. (1992) Nature 357:384-387	Male sterility	Counter clockwise
12	V-MCS-SYNTH	Synthetic	2	3370-3371
13	P-ta29-TOBAC	Nicotiana tabacum	1508	3372-4879	Promoter of theTA-29 gene. Seurinck et al. (1990). Nucleic Acid Res, 18(11), 3403		Counter clockwise
14	V-MCS-SYNTH	Synthetic	41	4880-4920
15	P-nos-RHIRD	Agrobacterium tumefaciens	294	4921-5214	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807		Clockwise
16	V-MCS-SYNTH	Synthetic	2	5215-5216
17	CS-barstar-BACAM	Bacillus amyloliquefaciens	273	5217-5489	Ribonuclease inhibitor gene barstar. Mariani et al. (1992) Nature 357:384-387	Fertility restoration	Clockwise
18	V-MCS-SYNTH	Synthetic	65	5490-5554
19	T-tr7-RHIRD	Agrobacterium tumefaciens	212	5555-5766	3' untranslated region (3' UTR) of the Agrobacterium transcript 7 T-DNA gene. Dhaese et al. (1983) EMBO Journal 2(3):419-426		Clockwise
20	V-MCS-SYNTH	Synthetic	74	5767-5840
21	V-LB-RHIRD	Agrobacterium tumefaciens	25	5841-5865

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

USDA-APHIS No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	USDA-APHIS	No

Detection Methods

27 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-PGS-bar-A2/PGS-bar-B2	construct-specific	Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3	Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13).	ring trial validation	national standard	1
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-B'nase-F-n4/BNaseR-n3	element-specific	CS-barnase	Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-BstarF-n2 /BstarR-n2	element-specific	CS-barstar	Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-SSuAra-F/P-SSuAra-R	element-specific	P-rbcS-ARATH	Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-TA29-F/P-TA29-R	element-specific	P-ta29-TOBAC	Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	3
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	3
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar)(Debode et al., 2016).	ring trial validation	ISO standard	1
QL-TAX-BN-003	taxon-specific	Brassica napus	Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-BN-004	taxon-specific	Brassica napus	Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-EVE-BN-011	event-specific	Ms11	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms11 (EU-RL GMFF GMOMETHODS database as of February 15, 2019).	ring trial validation	EU reference method	1
QT-TAX-BN-001	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014) Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-BN-002	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4	ring trial validation	EU reference method	1
QT-TAX-BN-003	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020)	ring trial validation	EU reference method	1
QT-TAX-BN-012	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-FatA primer1/FatA primer2	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	in-house validation	none	1

Description:	Ms11 was produced by means of Agrobacterium-mediated transformation using the vector pTC0113. Southern blot results demonstrated the presence of one complete T-DNA insert containing the bar, the barnase and the barstar gene cassettes in Ms11 B. napus. The hybrid technology comprises three components: a dominant gene for male sterility - the barnase gene (event Ms11), a dominant gene for fertility restoration - the barstar gene (event Rf3) and the bar gene (found in both Ms11 and Rf3) conferring tolerance to glufosinate-ammonium. Ms11 is a male sterile line that segregates 1:1 for sterility and fertility and is only used for the production of the Ms11xRf3 B. napus hybrid seed. According to Bayer, it will never be commercialized as a stand-alone product.
Links regarding to this GMO:	USDA-APHIS http://www.aphis.usda.gov/brs/aphisdocs/16_23501p.pdf
Transformation / Modification technique:	Agrobacterium-induced

Catalogue number	Source	Value	Type	Certified
AOCS 0306-B4	AOCS	<0.01 % (m/m)	genomic DNA	yes
AOCS 0306-B5	AOCS	<0.005 % (m/m)	genomic DNA	yes
AOCS 1116-A	AOCS	>99.99 % (m/m)	genomic DNA	yes

Continent	Country	File Reference	Intendend Use Details	Legal Status Details	Legislation
Europe	EU	EFSA-GMO-BE-2016-138	other uses - products other than food and feed containing or consisting of GMO for the same uses as any other of this species with the exception of cultivation	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	EFSA-GMO-BE-2016-138	feed - feed containing, consisting of, or produced from GMO	not approved - pending	Regulation (EC) No 1829/2003
Europe	EU	EFSA-GMO-BE-2016-138	cultivation - cultivation	not approved - not submitted	Regulation (EC) No 1829/2003
Europe	EU	EFSA-GMO-BE-2016-138	food - foods and food ingredients containing, consisting of, or produced from GMO	not approved - pending	Regulation (EC) No 1829/2003

Citation	Publication year	Publishing authority	Publication type	Content types
Statement complementing the EFSA Scientific Opinion on the assessment of genetically modified oilseed rape MS11 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 (application EFSA-GMO-BE-2016-138)	2022	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Assessment of genetically modified oilseed rape MS11 for food and feed uses, import and processing, under Regulation (EC) No 1829/2003 (application EFSA-GMO-BE-2016-138)	2020	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-011	2019	JRC	EU reference method protocol	detection
Request for Authorization of genetically modified male sterile and herbicide tolerant MS11 Brassica napus for food and feed uses, and import and processing, in accordance with articles 5 and 17 of Regulation (EC) N° 829/2003. EFSA-GMO-BE-2016-XXX. Part VII Summary.	2016	EFSA	application summary	application summary

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Company:

Developer:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference