Description:
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Cantaloupe lines A and B have been
genetically engineered to contain a
modified SAMase (sam-k) gene derived
from Escherichia coli bacteriophage T3.
The sam-k gene encodes an Sadenosylmethionine
hydrolase enzyme capable of degrading and thus reducing S-adenosylmethionine (SAM).
Reduction of SAM results in lowered ethylene production during fruit ripening in cantaloupe lines A and B and a corresponding increase in the
uniformity of ripening in the field.
The subject cantaloupe lines also contain and express the neomycin phosphotransferase II (nptII) gene derived from E. coli. The nptII gene was used as a selectable marker during the plant transformation process.
Expression of the added genes is controlled in part by gene sequences from the plant pathogen Agrobacterium tumefaciens, and the A. tumefaciens method was used to transfer the added genes into the parental inbred cantaloupe lines.
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Links regarding to this GMO:
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ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=235,
BCH http://bch.cbd.int/database/record.shtml?documentid=15389
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Transformation / Modification technique:
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Agrobacterium-induced
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