GMO molecular characterization details

GMO detailed information

GMO:
GTSB77
Alias:

former UID: SY-GTSB77-8
UID:
SY-GTSB77-7
Tradename:
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Beta vulgaris
(sugar beet)
Traits:
EU authorisation:

no Reference materials could be found
No results found.

General Information

Inserts / Modification

Name:	insert_GTSB77
Comment:
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens		-
2	P-34S FMV	Figwort mosaic virus		-	34S promoter derived from Figwort mosaic virus (FMV). Shepard et al. (1987) Phytopathology 77:1668-1673; Richins et al. (1987) Nucl Acids Res. 15:8451-8466; Gowda et al. (1989) J Cell Biochem. 13D (supplement):301
3	TP-ctp2-ARATH	Arabidopsis thaliana		-
4	CS-CP4epsps-RHIRD	Agrobacterium tumefaciens ssp. CP4		-	5-enolpyruvylshikimate- 3-phosphate synthase gene. Steinruecken et al. (1980) Biochem Biophys Res Com. 94:1207-1212	Glyphosate tolerance
5	T-rbcS_E9-PEA	Pisum sativum		-	3' untranslated region (3' UTR) f the rinulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) gene. Coruzzi et al. (1984) EMBO J. 3:1671-1679
6	P-e35S-CaMV	Cauliflower mosaic virus		-	Cauliflower mosaic virus (CaMV) 35S promoter with duplicated enhancer region; inserted element length can vary between e.g. 307bp/322bp/296bp/551bp/615bp; Kay R, et al. (1987) Science 236, 1299-1302
7	CS-uidA-ECOLX	Escherichia coli		-	uidA (gus) gene. Jefferson et al. (1987) The EMBO Journal 6(13):3901-3907	b-glucuronidase (GUS) activity
8	T-rbcS_E9-PEA	Pisum sativum		-	3' untranslated region (3' UTR) f the rinulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) gene. Coruzzi et al. (1984) EMBO J. 3:1671-1679
9	P-34S FMV	Figwort mosaic virus		-	34S promoter derived from Figwort mosaic virus (FMV). Shepard et al. (1987) Phytopathology 77:1668-1673; Richins et al. (1987) Nucl Acids Res. 15:8451-8466; Gowda et al. (1989) J Cell Biochem. 13D (supplement):301
10	TP-rbcS-ARATH	Arabidopsis thaliana		-
11	CS-gox-OCHAN	Ochrobactrum anthropi		-	Glyphosate oxidoreductase gene. Berry et al. (1995). US Patent 5.463.175	Glyphosate tolerance

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

application documents No

Detection Methods

19 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-CP1/pFMV2	construct-specific	GT200, GT73, GTSB77, H7-1, J101, J163, MON1445, MON1698, MON87705, MON88302, MON88913, MON89788, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative conventional PCR method for detection of the junction between the FMV promoter and the cp4-epsps gene (BVL G30.40-11).	ring trial validation	national standard	1
QL-CON-00-008	construct-specific	2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-epsps 1-5'/epsps 3-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-epsps2 1-5'/epsps2 1-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-PFMV 1-5'/PFMV 1-3'	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009)	in-house validation	none	1
QL-ELE-P-FMV-F/P-FMV-R	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-010	element-specific	E-FMV, P-34S FMV	Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-015	element-specific	E-FMV, P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5).	ring trial validation	ISO standard	2
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-019	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-024	element-specific	T-rbcS_E9-PEA	Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	2
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-TAX-BV-013	taxon-specific	Beta vulgaris	Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014)	ring trial validation	EU reference method	1

Authorisation

Literature reference