GMO molecular characterization details

GMO detailed information

GMO:
AM04-1020
Alias:
UID:
BPS-A1Ø2Ø-5
Tradename:

Amadea
Companies/Institutions:
BASF
Species:
Solanum tuberosum
(potato)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_AM04-1020
Comment:	2023-08-08 ED added AgroBorderI element; position unclear Revised according to FDA Biotechnology Consultation Note to the File BNF No. 000130 Application submitted under regulation (EC) No 1829/2003: EFSA-Q-2010-01138 on Part II - Summary of EFSA-GMO-SE-2010-88 http://registerofquestions.efsa.europa.eu/roqFrontend/?wicket:interface=:3:1::: Corresponding BCH Record ID: 48075 http://bch.cbd.int/database/record.shtml?documentid=48075
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens		-
2	P-gbss-SOLTU	Solanum tuberosum		-	Promoter of the granule-bound starch synthase gene. Van der Steege et al. (1992). Plant Mol Biol, 20(1):19-30		lenght: 999 bp
3	CS-gbss_RNAi_s-SOLTU	Solanum tuberosum		-	Fragment of the granule-bound starch synthase gene (gbss) in sense orientation	Amylose/amylopectin ratio	lenght: 457 bp; fragment of the gbss gene; generates with the corresponding antisense fragmenta double stranded RNA that triggers the degradation of gbss transcripts.
4	R-gbss_spacer-SOLTU	Solanum tuberosum		-			lenght: 66bp
5	CS-gbss_RNAi_as-SOLTU	Solanum tuberosum		-	Fragment of the granule-bound starch synthase gene (gbss) in antisense orientation	Amylose/amylopectin ratio	lenght: 457 bp; fragment of the gbss gene; generates with the corresponding sense fragmenta double stranded RNA that triggers the degradation of gbss transcripts.
6	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		lenght: 253 bp
7	P-nos-RHIRD	Agrobacterium tumefaciens		-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807		lenght: 288 bp
8	CS-ahas-ARATH	Arabidopsis thaliana		-	Acetohydroxy acid synthase gene (mutated) from Arabidopsis thaliana. Chang and Duggleby (1998). Biochem J, 333, 765-777	ALS/AHAS inhibitor tolerance	lenght: 2013 bp; encodes a mutant acetohydroxyacid synthase (S653N)
9	T-nos-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		truncated; deletion does not affect functionality
	V-Flanking Ti-nos-RHIRD	Agrobacterium tumefaciens		-	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the nopaline type; sequence located in the promoter of nopaline synthase.

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

application documents No

Detection Methods

11 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-TAX-ST-010	taxon-specific	Solanum tuberosum	Quantitative real-time PCR (TaqMan) method for detection of potato UDP-glucose pyrophosphorylase gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference