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Description:
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The maize event DAS40278 has been created by silicone carbide whiskers-mediated transformation with the 6236 bp transformation fragment from plasmid pDAS1740 carring the aad1 expression cassette.
The aad-1 gene (CS-aad1-SPHHE) encodes the aryloxyalkanoate dioxygenase (AAD-1) enzyme, which confers tolerance to 2,4-D and to certain aryloxyphenoxypropionate (AOPP) herbicides such as quizalofop.
The molecular characterization of the genetic modification in DAS40278 demonstrates that a
single copy of each of the genetic elements of the aad-1 expression cassette is present.
The inserted DNA fragment is stably integrated and inherited across and within breeding generations, and no plasmid backbone sequences are present in event DAS40278.
Sources:
- EFSA document: Application submitted under regulation (EC) No 1829/2003: EFSA-Q-2016-00674 on Part II - Summary of EFSA-GMO-NL-2010-89
- USDA-APHIS petition 09-233-01p
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Links regarding to this GMO:
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BCH http://bch.cbd.int/database/record.shtml?documentid=104814,
ISAAA http://www.isaaa.org/gmapprovaldatabase/event/default.asp?EventID=139,
USDA-APHIS petition https://www.aphis.usda.gov/brs/aphisdocs/09_23301p.pdf
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Transformation / Modification technique:
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whiskers-mediated
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