MZHG0JG maize was produced by Agrobacterium tumefaciens–mediated transformation of immature embryos of Syngenta’s proprietary maize inbred line NP2222.

Genetic characterization studies demonstrated that MZHG0JG maize contains, at a single locus within the maize genome, a single copy of each of the following functional elements: CS-mepsps, CS-pat, E-FMV, E-35S, TP-OTP, P-Ubi, E-TMV, T-Ubi, P-35S, and T-nos.

No extraneous DNA fragments of these functional elements occur elsewhere in the MZHG0JG maize genome. Similarly, plasmid backbone sequence from transformation plasmid pSYN18857 is not present in the MZHG0JG maize genome.

Southern blot analyses demonstrated that the MZHG0JG maize T-DNA insert is stably inherited from one generation to the next and that the MZHG0JG maize genome contains a single T-DNA insert. Nucleotide sequence analysis determined that the MZHG0JG insert consists of the intact T-DNA region of the pSYN18857 plasmid vector.