GMO molecular characterization details

GMO detailed information

GMO:
EF2-114
Alias:
UID:
FDP-ØØ114-5
Tradename:

Rosé, PinkGlow
Companies/Institutions:
Del Monte Fresh Produce
Species:
Ananas comosus
(Pineapple)
Traits:
EU authorisation:

no Methods could be found.
no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert1_EF2-114 (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the transformation vector pHCWflACC3ʹ-2. Order of cassettes according to patent US 2013/0326768, fig. 3. Source: Patent US 2013/0326768 ; FDA Biotechnology Consultation.
Map:

Genetic Elements:

Reference: FDA

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	-			lenght: 25 bp
2	V-MCS-SYNTH	Synthetic	-			lenght: 110 bp
3	P-ephx-ANACO	Ananas comosus	-	promoter of the epoxide hydrolase gene from Ananas. Constitutive expression; length: 1.7 kb		Constitutive expression; lenght: 1.7 kb
4	P-ubi-ANACO	Ananas comosus	-	promoter of the tetrameric ubiquitin (Ubi) gene from Ananas, including endogenous intron; length: 1,5 kb + 1.057 kb for the endogenous intron.		Constitutive expression. Includes endogenous intron; lenght: 1.5 kb + 1.057 kb for the endogenous intron.
5	CS-SuRB-TOBAC	Nicotiana tabacum	-	Acetohydroxy acid synthase gene (SuRB). Mazur et al. (1987). Plant Physiol 85, 1110-1117	Sulfonylurea tolerance	lenght: 1.17 kb
6	T-SuRB-TOBAC	Nicotiana tabacum	-	3' untranslated region (3' UTR) of the Nicotiana tabacum Acetohydroxy acid synthase gene. Mazur et al. (1987) Plant Physiol, 85(4): 1110-1117		lenght: 2.7 kb
7	P-ubi-ANACO	Ananas comosus	-	promoter of the tetrameric ubiquitin (Ubi) gene from Ananas, including endogenous intron; length: 1,5 kb + 1.057 kb for the endogenous intron.		Constitutive expression. Includes endogenous intron; lenght: 1.5 kb + 1.057 kb for the endogenous intron.
8	CS-acs_RNAi_s-ANACO	Ananas comosus	-	406 bp 3' sequence of meristem-specifc 1-aminocyclopropane-1-carboxylic acid (ACC)synthase gene, in the sense orientation.	Reduced ethylene synthesis	406 bp fragment of the acs gene in sense orientation that generates with the corresponding fragment in antisense orientation a double stranded RNA that triggers the degradation of acs transcripts to reduce etylene production and delayed flowering.
9	I-2_psbr-SOLTU	Solanum tuberosum	-	Intron 2 of the psbr gene (also known as light-inducible tissue-specific LS1 gene); length: 193bp.		193 bp spacer between sense and antisense gene fragments enhancing vector stability.
10	CS-acs_RNAi_as-ANACO	Ananas comosus	-	406 bp 3' sequence of meristem-specifc 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene, in the antisense orientation	Reduced ethylene synthesis	406 bp fragment of the acs gene in antisense orientation that generates with the corresponding fragment in sense orientation a double stranded RNA that triggers the degradation of acs transcripts to reduce etylene production and delayed flowering.
11	T-ubi-ANACO	Ananas comosus	-	3' untranslated region (3' UTR) and flanking region of the ubiquitin gene; lenght: 940 bp. Firoozbadly et al. (2014) US PP25,763 P3		length: 940 bp
12	V-MCS-SYNTH	Synthetic	-			lenght: 110 bp
13	V-LB-RHIRD	Agrobacterium tumefaciens	-			lenght: 25 bp

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

FDA No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	FDA	No

Name:	insert2_EF2-114 (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the transformation vector pHCW.T-7. Order of cassettes according to patent US 2013/0326768, fig. 3. Source: Patent US 2013/0326768 ; FDA Biotechnology Consultation.
Map:

Genetic Elements:

Reference: FDA

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	-			lenght: 25 bp
2	V-MCS-SYNTH	Synthetic	-			lenght: 110 bp
3	P-ephx-ANACO	Ananas comosus	-	promoter of the epoxide hydrolase gene from Ananas. Constitutive expression; length: 1.7 kb		Constitutive expression; lenght: 1.7 kb
4	P-ubi-ANACO	Ananas comosus	-	promoter of the tetrameric ubiquitin (Ubi) gene from Ananas, including endogenous intron; length: 1,5 kb + 1.057 kb for the endogenous intron.		Constitutive expression. Includes endogenous intron; lenght: 1.5 kb + 1.057 kb for the endogenous intron.
5	CS-SuRB-TOBAC	Nicotiana tabacum	-	Acetohydroxy acid synthase gene (SuRB). Mazur et al. (1987). Plant Physiol 85, 1110-1117	Sulfonylurea tolerance	lenght: 1.17 kb
6	T-SuRB-TOBAC	Nicotiana tabacum	-	3' untranslated region (3' UTR) of the Nicotiana tabacum Acetohydroxy acid synthase gene. Mazur et al. (1987) Plant Physiol, 85(4): 1110-1117		lenght: 2.7 kb
7	P-bi-ANACO	Ananas comosus	-	modified promoter derived from the pineapple bromelain inhibitor gene (bi); length: 2.5 kb. Sawano Y. et al. (2001). Journal of Biol. Chem. 227 (31), 28222-28227		lenght: 2.5 kb
8	CS-psy-CITUN	Citrus unshiu	-	Phytoene synthase gene	Altered caroteinoid biosynthesis	lenght: 1.131 kb
9	T-ubi-ANACO	Ananas comosus	-	3' untranslated region (3' UTR) and flanking region of the ubiquitin gene; lenght: 940 bp. Firoozbadly et al. (2014) US PP25,763 P3		lenght: 940 bp
10	P-bi-ANACO	Ananas comosus	-	modified promoter derived from the pineapple bromelain inhibitor gene (bi); length: 2.5 kb. Sawano Y. et al. (2001). Journal of Biol. Chem. 227 (31), 28222-28227		lenght: 2.5 kb
11	CS-lycb_RNAi_s-ANACO	Ananas comosus	-	619 bp fragment of the lycopene beta-cyclase (lycb) gene in sense orientation. The lycb gene encodes an enzyme that converts lycopene to gamma-carotene, a metabolic precursor of beta-carotene.	Altered caroteinoid biosynthesis	619 bp fragment of the lycb gene in sense orientation that generates with the corresponding fragment in antisense orientation a double stranded RNA that triggers the degradation of lycb transcripts to impair the lycopene metabolization.
12	I-2_psbr-SOLTU	Solanum tuberosum	-	Intron 2 of the psbr gene (also known as light-inducible tissue-specific LS1 gene); length: 193bp.		193 bp spacer between sense and antisense gene fragments enhancing vector stability.
13	CS-lycb_RNAi_as-ANACO	Ananas comosus	-	619 bp fragment of the lycopene beta-cyclase (lycb) gene in antisense orientation. The lycb gene encodes an enzyme that converts lycopene to gamma-carotene, a metabolic precursor of beta-carotene.	Altered caroteinoid biosynthesis	619 bp fragment of the lycb gene in antisense orientation that generates with the corresponding fragment in sense orientation a double stranded RNA that triggers the degradation of lycb transcripts to impair the lycopene metabolization.
14	T-ubi-ANACO	Ananas comosus	-	3' untranslated region (3' UTR) and flanking region of the ubiquitin gene; lenght: 940 bp. Firoozbadly et al. (2014) US PP25,763 P3		lenght: 940 bp
15	P-bi-ANACO	Ananas comosus	-	modified promoter derived from the pineapple bromelain inhibitor gene (bi); length: 2.5 kb. Sawano Y. et al. (2001). Journal of Biol. Chem. 227 (31), 28222-28227		lenght: 2.5 kb
16	CS-lyce_RNAi_s-ANACO	Ananas comosus	-	504 bp fragment of the lycopene epsilon-cyclase gene (lyce) in sense orientation. The lyce gene encodes an enzyme that converts lycopene to sigma-carotene, a metabolic precursor of alpha-carotene.	Altered caroteinoid biosynthesis	504 bp fragment of the lyce gene in sense orientation that generates with the corresponding fragment in antisense orientation a double stranded RNA that triggers the degradation of lyce transcripts to impair the lycopene metabolization.
17	I-2_psbr-SOLTU	Solanum tuberosum	-	Intron 2 of the psbr gene (also known as light-inducible tissue-specific LS1 gene); length: 193bp.		193 bp spacer between sense and antisense gene fragments enhancing vector stability.
18	CS-lyce_RNAi_as-ANACO	Ananas comosus	-	504 bp fragment of the lycopene epsilon-cyclase gene (lyce) in antisense orientation. The lyce gene encodes an enzyme that converts lycopene to sigma-carotene, a metabolic precursor of alpha-carotene.	Altered caroteinoid biosynthesis	504 bp fragment of the lyce gene in antisense orientation that generates with the corresponding fragment in sense orientation a double stranded RNA that triggers the degradation of lyce transcripts to impair the lycopene metabolization.
19	T-ubi-ANACO	Ananas comosus	-	3' untranslated region (3' UTR) and flanking region of the ubiquitin gene; lenght: 940 bp. Firoozbadly et al. (2014) US PP25,763 P3		lenght: 940 bp
20	V-MCS-SYNTH	Synthetic	-			lenght: 110 bp
21	V-LB-RHIRD	Agrobacterium tumefaciens	-			lenght:25 bp

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

FDA No

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

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