GMO molecular characterization details

GMO detailed information

GMO:
DAS81419
Alias:
UID:
DAS-81419-2
Tradename:

Conkesta
Companies/Institutions:
DOW AgroSciences
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

Inserts / Modification

Name:	insert_DAS81419
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-ubi10-ARATH	Arabidopsis thaliana		-	Promoter of the Ubiquitin-10 gene. Norris et al. (1993). Plant Mol Biol, 21(5):895-906
2	CS-cry1Fa2_1Ca3_1Ab1-SYNTH	Synthetic		-	plant-optimized gene coding for a full length chimeric version of Cry1F originally from Bt. ssp. Aizawai (toxin core from Cry1Fa2, very small portion of Cry1Ca3 and C-terminal sequence from Cry1Ab1); Cardineau et al., 2001; Gao et al., 2006.	Lepidoptera resistance	Derivative of CS-cry1F-BACTA which is a chimeric version of the native CS-cry1F-BACTA (cry1Fa2), CS-cry1Ca3-BACTA (short sequence stretch in the middle of protein) and CS-cry1Ab1-BACTB (codes for tail at the 3´ end of the gene) that results in a 1148 amino acid-comprising protein.
3	T-orf23-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the ORF23 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350
4	P-CsVMV	Cassava vein mosaic virus		-	Promoter region of the Cassava vein mosaic virus. Verdaguer et al. (1996). Plant Mol Biol, 31:1129-1139.
5	CS-cry1Ac1_1Ca3_1Ab-SYNTH	Synthetic		-	plant-optimized gene coding for a full-length chimeric version of Cry1Ac1, originally from Bt ssp. Kurstaki HD73 (toxin core from Cry1Ac1; and C-terminal sequences from Cry1Ca3 from Bt. ssp Aizawai PS81I and Cry1Ab1 from Bt. ssp. Berliner 1715).	Lepidoptera resistance	Derivative of CS-cry1Ac-BACTK (cry1Ac1) which is a chimeric version of the native CS-cry1Ac-BACTK, CS-cry1Ca3-BACTA (short sequence stretch in the middle of protein) and CS-cry1Ab1- BACTB (codes for tail at the 3´ end of the gene) that results in a 1156 amino acid-comprising protein.
6	T-orf23-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the ORF23 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350
7	P-CsVMV	Cassava vein mosaic virus		-	Promoter region of the Cassava vein mosaic virus. Verdaguer et al. (1996). Plant Mol Biol, 31:1129-1139.
8	CS-pat-STRVR	Streptomyces viridochromogenes		-	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37	Glufosinate tolerance
9	T-orf1-RHIRD	Agrobacterium tumefaciens		-	3' untranslated region (3' UTR) of the ORF1 from Agrobacterium tumefaciens Ti-Plasmid pTi15955. Barker et al. (1983) Plant Mol Biol. 2(6):335-350

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
HW660956	NCBI - GenBank	Yes	15294	SOYBEAN EVENT pDAB9582.814.19.1 DETECTION METHOD, sequence of event DAS81419 (including amplicon for method QT-EVE-GM-014)
RIKILT_DAS81419_event	Wageningen Food Safety Research (WFSR)	Yes	104	Amplicon sequence of DAS81419 for event-specific method QT-EVE-GM-014 without probe

Detection Methods

18 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-CsVMV F/patsyn R	construct-specific	DAS44406, DAS68416, DAS81419	Qualitative real-time PCR (TaqMan) method for detection of the junction between the Cassava vein mosaic virus promoter (P-CsVMV) and the phosphinothricin N-acetyltransferase (pat) gene. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	3
QL-ELE-00-021	element-specific	CS-pat-STRVR	Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-025	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.	ring trial validation	EU reference method	3
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-ELE-00-002	element-specific	CS-pat-STRVR	Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)	ring trial validation	unknown	2
QT-EVE-GM-014	event-specific	DAS81419	Quantitative PCR method for detection of soybean event DAS81419 (EURL GMFF GMOMETHODS database as of May 28, 2015)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference