GMO molecular characterization details

GMO detailed information

GMO:
LLCotton25
Alias:
UID:
ACS-GHØØ1-3
Tradename:

LibertyLink
Companies/Institutions:
Bayer CropScience, Aventis CropScience
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_LLcotton25
Comment:
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	-			approximate size of element: 30 bp; Right border repeat from the TL-DNA from pTiB6S3 of Agrobacterium tumefaciens, strain B6S3 (Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell, J (1984) The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5, The EMBO Journal, 3, 835-846)
2	V-MCS-SYNTH	Synthetic	-			approximate size of element: 30 bp
3	P-35S-CaMV	Cauliflower mosaic virus	-			approximate size of element: 1390 bp
4	CS-bar-STRHY	Streptomyces hygroscopicus	-	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523	Glufosinate tolerance	approximate size of element: 550 bp
5	V-MCS-SYNTH	Synthetic	-			approximate size of element: 20 bp
6	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807		approximate size of element: 260 bp
7	V-MCS-SYNTH	Synthetic	-			approximate size of element: 50 bp
8	V-LB-RHIRD	Agrobacterium tumefaciens	-			approximate size of element: 30 bp; Left border repeat from the TL-DNA from pTiB6S3 of Agrobacterium tumefaciens, strain B6S3 (Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M, Van Montagu M, Schell, J (1984) The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5, The EMBO Journal, 3, 835-846)

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	application documents	No
RIKILT_LLcotton25_event	Wageningen Food Safety Research (WFSR)	Yes	79	Amplicon sequence of LLcotton25 for event-specific method QT-EVE-GH-002 without probe

Detection Methods

27 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-015	construct-specific	Bt176, CBH351, DBT418, DLL25, EH-2 modbs 2.99, IE034, Iksan515, Iksan526, LLCotton25, LLRICE06, LLRICE601, LLRICE604, LLRICE62, MON88701, Ms3, Ms6, ND207, SYHT04R, T1c-19, T2A-1, T303-3, T304-40, TC6275, Varuna bn 3.6, Verdeca HB4, W62, W98	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the bar gene (Bayer CropScience, 2006; verified by the EU-RL GMFF in the context of Commission Decision 2006/578/EC)	in-house validation	none	2
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	3
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QT-EVE-GH-002	event-specific	LLCotton25	Quantitative real-time PCR (TaqMan) method for detection of cotton event LLCotton25 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)	ring trial validation	EU reference method	3
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	3
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Catalogue number	Source	Value	Type	Certified	Available
AOCS 0306-A3	AOCS	<0.01 % (m/m)	genomic DNA	yes	yes
AOCS 0306-E	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-E2	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-E3	AOCS	>99.99 % (m/m)	genomic DNA	yes	yes
5211512401	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Commission decision number	Expiry date
1	EFSA-GMO-NL-2005-13	Gossypium hirsutum (cotton)	LLCotton25	food feed cultivation other	BASF Agricultural Solutions Seed US		28.10.2018
2	EFSA-GMO-RX-010	Gossypium hirsutum (cotton)	LLCotton25	food feed cultivation other	Bayer CropScience		27.11.2029

Citation	Publication year	Publishing authority	Publication type	Content types
Opinion of the Scientific Panel on Genetically Modified Organisms on an application (Reference EFSA-GMO-NL-2005-13) for the placing on the market of glufosinate-tolerant genetically modified LLCotton25, for food and feed uses, and import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience1 (Question No EFSA-Q-2005-047) Opinion adopted on 6 December 2006	2006	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No. 1829/2003 on application EFSA-GMO-NL-2005-13 Application for the placing on the market of glufosinate-tolerant genetically modified LLcotton25 for food and feed uses, import and processing from Bayer CropScience (Question No. EFSA-Q-2005-047)	2007	EFSA	EFSA overall opinion	risk assessment document
Mazzara M., Grazioli E., Savini C., Van Den Eede G.; Event-Specific Method for the Quantification of Cotton Line LLCotton25" Using Real-time PCR - Validation Report and Protocol - Cotton Seeds Sampling and DNA Extraction" Online Publication (2007)	2007		EU reference method protocol	detection
Part II Summary of the application for request the authorisation Glufosinate-ammonium tolerant cotton LLCotton25 for food and feed uses, and import and processing, in accordance with articles 5 and 17 of regulation 1829/2003 GM food and GM feed, and for industrial uses	2008	EFSA	application summary	application summary
COMMISSION DECISION of 29 October 2008 authorising the placing on the market of products containing, consisting of, or produced from genetically modified LLCotton25 (ACS-GHØØ1-3) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document number C(2008) 6204) (2008/837/EC)	2008	EU	EU Commission Decision	decision document
Request for Renewal of the Authorisation of the genetically modified herbicide tolerant cotton LLCotton25 for food and feed uses, import and processing, in accordance with Articles 11 and 23 of Regulation (EC) No 1829/2003	2017	EFSA	application summary	application summary
Assessment of genetically modified LLCotton25 for renewal of authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-010)	2018	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority on genetically modified LLCotton25 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-010)	2018	EFSA	EFSA overall opinion	risk assessment document
COMMISSION IMPLEMENTING DECISION (EU) 2019/2082 of 28 November 2019 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified cotton LLCotton25 (ACS-GHØØ1-3) pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2019) 7481)	2019	EU	EU Commission Decision	decision document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference