GMO molecular characterization details

GMO detailed information

GMO:
DP356043
Alias:

Event 3560.4.3.5, 356043
UID:
DP-356Ø43-5
Tradename:

GAT, Optimum
Companies/Institutions:
DuPont, Pioneer Hi-Bred International
Species:
Glycine max
(soybean)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_DP356043
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	P-scp1-SYNTH	Synthetic		-	Constitutive synthetic promoter comprising a portion of the CaMV 35S promoter and the Rsyn7-Syn II Core consensus promoter. U.S. patent 6,555,673. Bowen et al. 2003.
2	L-omega-TMV	Tobacco mosaic virus		-
3	CS-gat-BACLI	Bacillus licheniformis		-	Glyphosate-N-Acteyltransferase gene. Castel et al. (2004). Science, 304, 1151-1154	Glyphosate tolerance
4	T-pinII-SOLTU	Solanum tuberosum		-	3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence.
5	P-sams-SOYBN	Glycine max		-	Promoter of the S-adenosyl-L-methionine synthetase gene. Falco and Li (2007). Patent: US 7,217,858 B2
6	CS-ahas-SOYBN	Glycine max		-	Herbicide-resistant acetohydroxy acid synthase gene (mutated). Sebastian et al. (1989). Crop Sci, 29, 1403-1408; Sebastian (1992). US5084082A; Tan et al. (2005). Pest manag Sci, 61, 246-257	Sulfonylurea tolerance
7	T-ahas-SOYBN	Glycine max		-	3' untranslated region (3' UTR) of the native acetohydroxy acid synthase gene. Ghio et al. (2013) Theor Appl Genet 126:2957-2968

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
FB727428	NCBI - GenBank	Yes	10849	Sequence 6 from Patent WO2008002872, sequence of event DP356043 (including amplicon for method QT-EVE-GM-009)
FB727463	NCBI - GenBank	Yes	8679	Sequence 41 from Patent WO2008002872, left border sequence and complete transgene insert for event 3560.4.3.5
GY272890	NCBI - GenBank	Yes	5362	Sequence 3 from patent US 7968770, transgene insert for event 3560.4.3.5
RIKILT_DP356043_event	Wageningen Food Safety Research (WFSR)	Yes	99	Amplicon sequence of DP356043 for event-specific method QT-EVE-GM-009 without probe

Detection Methods

15 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-gat/T-pinII-F/gat/T-pinII-R	construct-specific	61061, 73496, 98140, DP356043	Qualitative real-time PCR (TaqMan) method for detection of the junction between the gat gene and pinII terminator (Prins et al., 2016)	in-house validation	national standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-TAX-GM-001	taxon-specific	Glycine max	Qualitative real-time PCR (SYBRGreen) method for detection of soybean lectin gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-GM-002	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO/FDIS 21569).	ring trial validation	ISO standard	1
QL-TAX-GM-008	taxon-specific	Glycine max	Qualitative conventional PCR method for detection of soybean lectin gene (ISO 21569)	ring trial validation	ISO standard	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-GM-009	event-specific	DP356043	Quantitative real-time PCR (TaqMan) method for detection of soybean event DP356043 (EU-RL GMFF GMOMETHODS database as of January 23, 2014)	ring trial validation	EU reference method	3
QT-TAX-GM-001	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-002	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-GM-003	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GM-004	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene (Hird et al., 2003).	ring trial validation	unknown	1
QT-TAX-GM-005	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) for detection of soybean lectin gene (EU-RL GMFF GMOMETHODS database as of November 8, 2023).	ring trial validation	EU reference method	1
QT-TAX-GM-007	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin gene	ring trial validation	ISO standard	1
QT-TAX-GM-009	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014)	ring trial validation	EU reference method	1
QT-TAX-GM-020	taxon-specific	Glycine max	Quantitative real-time PCR (TaqMan) method for detection of soybean lectin (Le1) gene (EU-RL GMFF GMOMETHODS database as of January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1

Reference Materials

Authorisation

Literature reference