GMO molecular characterization details

GMO detailed information

GMO:
Ms8
Alias:
UID:
ACS-BNØØ5-8
Tradename:

InVigor
Companies/Institutions:
Aventis CropScience, Bayer CropScience
Species:
Brassica napus
(canola, oilseed rape, rapeseed)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_Ms8
Comment:	T-DNA as described in plasmid pTHW107 used for transformation.
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	-			element length: 25 bp
2	V-MCS-SYNTH	Synthetic	-			element length: 72 bp
3	T-tr7-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the Agrobacterium transcript 7 T-DNA gene. Dhaese et al. (1983) EMBO Journal 2:419-426.		element length: 212 bp
4	V-MCS-SYNTH	Synthetic	-			element length: 21 bp
5	CS-bar-STRHY	Streptomyces hygroscopicus	-	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523.	Glufosinate tolerance	element length: 552 bp
6	P-rbcS-ARATH	Arabidopsis thaliana	-	Promoter of the ribulose- 1,5-bisphosphate carboxylase (Rubisco) small subunit gene. Almeida et al. (1989) Mol Gen Genet, 218:78-86.		element length: 1726 bp
7	V-MCS-SYNTH	Synthetic	-			element length: 50 bp
8	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807.		element length: 261 bp
9	T-barnase-BACAM	Bacillus amyloliquefaciens	-	3' untranslated region (3' UTR) of the extracellular ribonuclease (barnase) gene. An inverted repeat at the 3' end of the gene may act as a transcription terminator. Paddon and Hartley (1986) Gene 40:231-239.		element length: 112 bp
10	CS-barnase-BACAM	Bacillus amyloliquefaciens	-	Ribonuclease gene barnase. Mariani et al. (1992) Nature 357:384-387.	Male sterility	element length: 336 bp
11	P-ta29-TOBAC	Nicotiana tabacum	-	Promoter of theTA-29 gene. Seurinck et al. (1990). Nucleic Acid Res, 18(11), 3403.		element length: 1510 bp
12	V-MCS-SYNTH	Synthetic	-			element length: 44 bp
13	V-LB-RHIRD	Agrobacterium tumefaciens	-			element length: 25 bp

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	application documents	No
GV638084	NCBI - GenBank	Yes	537	Sequence 18 from patent US 7659095, sequence of event Ms8 (including amplicon for method QT-EVE-BN-002)
GV638104	NCBI - GenBank	Yes	522	Sequence 38 from patent US 7659095, sequence of event Ms8 (including amplicon for method QT-EVE-BN-002)
RIKILT_Ms8_event	Wageningen Food Safety Research (WFSR)	Yes	130	Amplicon sequence of Ms8 for event-specific method QT-EVE-BN-002 without probe
RIKILT_P-TA29_element_Ms8	Wageningen Food Safety Research (WFSR)	Yes	117	Amplicon sequence of P-TA29 for element-specific method QL-ELE-P-TA29-F/P-TA29-R without probe
RIKILT_barnase_element_Ms8	Wageningen Food Safety Research (WFSR)	Yes	102	Amplicon sequence of barnase for element-specific method QL-ELE-B'nase-F-n4/BNaseR-n3 without probe

Detection Methods

24 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-PGS-bar-A2/PGS-bar-B2	construct-specific	Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)	Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13).	ring trial validation	national standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	2
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	2
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	3
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	3
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QL-ELE-B'nase-F-n4/BNaseR-n3	element-specific	CS-barnase	Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).	in-house validation	none	3
QL-ELE-P-SSuAra-F/P-SSuAra-R	element-specific	P-rbcS-ARATH	Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-TA29-F/P-TA29-R	element-specific	P-ta29-TOBAC	Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013; Official Collection of Methods according to § 28b GenTG, G 30.40-22)	ring trial validation	national standard	3
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	3
QL-TAX-BN-003	taxon-specific	Brassica napus	Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-BN-004	taxon-specific	Brassica napus	Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	3
QT-EVE-BN-002	event-specific	Ms8	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms8 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	3
QT-TAX-BN-001	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014) Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-BN-002	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4	ring trial validation	EU reference method	1
QT-TAX-BN-003	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020)	ring trial validation	EU reference method	1
QT-TAX-BN-012	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-FatA primer1/FatA primer2	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	in-house validation	none	1

Reference Materials

Catalogue number	Source	Value	Type	Certified	Available
5211507901	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no
5211513702	Eurofins GeneScan	20 cp/µl	genomic DNA	no	no
5211513720	Eurofins GeneScan	20 cp/µl	genomic DNA	no	no
AOCS 0306-B3	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0306-B4	AOCS	<0.01 % (m/m)	genomic DNA	yes	no
AOCS 0306-B5	AOCS	<0.005 % (m/m)	genomic DNA	yes	yes
AOCS 0306-F2	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-F3	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-F5	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-F7	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-F8	AOCS	>99.99 % (m/m)	genomic DNA	yes	no
AOCS 0306-F9	AOCS	>99.99 % (m/m)	genomic DNA	yes	yes

Authorisation

UID

Decision Date

Legislation

Traits

Genetic Elements

Tradenames

Companies/Institutions

Your Search criteria is: ( GMO: Ms8 ) AND ( country: EU ) Refine Search

	Application reference number	Species	GMO	Intended use and EU authorisation status	Applicant	Commission decision number	Expiry date
1	EFSA-GMO-RX-024	Brassica napus (canola, oilseed rape, rapeseed)	Ms8	food feed cultivation other	BASF Agricultural Solutions Seed US	(EU) 2024/389	25.01.2034
2	EFSA-GMO-RX-004	Brassica napus (canola, oilseed rape, rapeseed)	Ms8	food feed cultivation other	BASF Agricultural Solutions Seed US	(EU) 2019/1301, 2013/327/EU	24.06.2023
3	EFSA-GMO-BE-2010-81	Brassica napus (canola, oilseed rape, rapeseed)	Ms8	food feed cultivation other	BASF Agricultural Solutions Seed US	(EU) 2019/1301, 2013/327/EU	24.06.2023
4	EFSA-GMO-RX-Ms8xRf3-(8.1a/20.1b)	Brassica napus (canola, oilseed rape, rapeseed)	Ms8	food feed cultivation other	BASF Agricultural Solutions Seed US	2013/327/EU	24.06.2023
5	C/BE/96/01	Brassica napus (canola, oilseed rape, rapeseed)	Ms8	food feed cultivation other	Bayer CropScience	(EU) 2019/1301, 2007/232/EC	26.07.2019

Citation	Publication year	Publishing authority	Publication type	Content types
Commission Implementing Decision (EU) 2024/389 of 26 January 2024 renewing the authorisation for the placing on the market of products containing, consisting of or produced from genetically modified oilseed rape Ms8, Rf3 and Ms8 × Rf3 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council	2024	EU	EU Commission Decision	decision document
Assessment of genetically modified oilseed rape MS8, RF3 and MS8 x RF3 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-024)	2023	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Request for Renewal of the Authorization of the genetically modified herbicide tolerant canola Ms8, Rf3 and Ms8 x Rf3 for food and feed uses, and import and processing, in accordance with articles 11 and 23 of Regulation (EC) No 1829/2003, EFSA-GMO-RX024, Part III - Summary	2021	EFSA	application summary	application summary
Commission Implementing Decision (EU) 2019/1301 of 26 July 2019 amending Implementing Decision 2013/327/EU as regards the renewal of the authorisation to place on the market feed containing or consisting of genetically modified oilseed rapes Ms8, Rf3 and Ms8 × Rf3 pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2019) 5499) (Text with EEA relevance.) C/2019/5499	2019	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority on genetically modified oilseed rape MS8, RF3 and MS8×RF3 for renewal authorisation under Regulation (EC) No 1829/2003 (application EFSA-GMO-RX-004)	2017	EFSA	EFSA overall opinion	risk assessment document
Assessment of genetically modified oilseed rape MS8, RF3 and MS8xRF3 for renewal of authorisation under regulation (EC) No 1829/2003 (application EFSA-GMO-RX-004)	2017	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
PCR detection of the pSSUAra-bar construct for screening of genetically modified oilseed rape lines - construct-specific method. BVL Official Method Collection G 30.40-13 Online Publication (2015)	2015		other document	detection
Commission implementing decision of 25 June 2013 authorising the placing on the market of food containing or consisting of genetically modified oilseed rape Ms8, Rf3 and Ms8 × Rf3, or food and feed produced from those genetically modified organisms pursuant to Regulation (EC) No 1829/2003 of the European Parliament and of the Council (notified under document C(2013) 3873) (2013/327/EU)	2013	EU	EU Commission Decision	decision document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on application (reference EFSA-GMO-BE-2010-81) for the placing on the market of the genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3 for food containing or consisting of, and food produced from or containing ingredients produced from genetically modified oilseed rape Ms8, Rf3 and Ms8 x Rf3 (with the exception of processed oil) under Regulation (EC) No 1829/2003 from Bayer European Food Safety Authority	2012	EFSA	EFSA overall opinion	risk assessment document
Request for Authorization of Glufosinate Ammonium-Tolerant Genetically Modified Oilseed Rape Ms8, Rf3 and Ms8xRf3 in accordance with article 5 of Regulation (EC) No 1829/2003. Part II - Summary	2012	EFSA	application summary	application summary
Scientific Opinion on application (EFSA-GMO-BE-2010-81) for the placing on the market of genetically modified herbicide-tolerant oilseed rape Ms8, Rf3 and Ms8 × Rf3 for food containing or consisting of, and food produced from or containing ingredients produced from, oilseed rape Ms8, Rf3 and Ms8 × Rf3 (with the exception of processed oil) under Regulation (EC) No 1829/2003 from Bayer EFSA Panel on Genetically Modified Organisms (GMO)	2012	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Scientific Opinion on the revised molecular characterisation for Rf3 oilseed rape received from the Competent Authority of Belgium under Article 20(3) of Directive 2001/18/EC1 (Application C/BE/96/01)	2010	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
Overall opinion of the European Food Safety Authority in accordance with Articles 6 and 18 of Regulation (EC) No 1829/2003 on the application (reference EFSA-GMO-RX-Ms8-Rf3) for renewal of the authorisation of existing products produced from herbicide tolerant genetically modified oilseed rape Ms8 x Rf3 for food and feed uses from Bayer CropScience AG European Food Safety Authority	2009	EFSA	EFSA overall opinion	risk assessment document
Summary of the request for renewal, according to Articles 11 and 23, of the authorisations notified under Articles 8(1)(A) and 20(1)(B) of Regulation (EC) No. 1829/2003 - Glufosinate ammonium-tolerant genetically modified oilseed rape Ms8/Rf3 for derived food and feed products Part II - Summary	2008	EFSA	application summary	application summary
Mazzara M., Bogni A., Savini C., Van Den Eede G.; Event-specific Method for the Quantification of Oilseed Rape Line Ms8 Using Real-time PCR - Validation Report and Protocol- Seeds Sampling and DNA Extraction of Oilseed Rape Online Publication (2007)	2007		EU reference method protocol	detection
Commission Decision of 26 March 2007 concerning the placing on the market, in accordance with Directive 2001/18/EC of the European Parliament and of the Council, of oilseed rape products (Brassica napus L., lines Ms8, Rf3 and Ms8xRf3) genetically modified for tolerance to the herbicide glufosinate-ammonium, 2007/232/EC	2007	EU	EU Commission Decision	decision document
Opinion of the Scientific Panel on Genetically Modified Organisms on a request from the Commission related to the application (Reference C/BE/96/01) for the placing on the market of glufosinate-tolerant hybrid oilseed rape Ms8 x Rf3, derived from genetically modified parental lines (Ms8, Rf3), for import and processing for feed and industrial uses, under Part C of Directive 2001/18/EC from Bayer CropScience (Question No EFSA-Q-2005-003)	2005	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference