GMO molecular characterization details

GMO detailed information

GMO:
DP202216
Alias:
UID:
DP-2Ø2216-6
Tradename:
Companies/Institutions:
Pioneer Hi-Bred International
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

General Information

Inserts / Modification

Name:	insert_DP202216
Comment:
Map:

Genetic Elements:

Reference: patent documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-RB-RHIRD	Agrobacterium tumefaciens	25	1-25
2	V-Flanking Ti-nos-RHIRD	Agrobacterium tumefaciens	113	65-177	Flanking sequence from Agrobacterium tumefaciens Ti plasmids of the nopaline type; sequence located in the promoter of nopaline synthase.
3	V-intervening-SYNTH	Synthetic	258	178-435
4	V-loxP-BPP1	Bacteriophage P1	34	436-469			Bacteriophage P1 recombination site recognized by Cre recombinase (loxP)
5	V-intervening-SYNTH	Synthetic	229	470-698
6	V-intervening sequences		21	699-719			Bacteriophage lambda integrase recombination site (attB4)
7	V-intervening-SYNTH	Synthetic	34	720-753
8	P-gos2-MAIZE	Zea mays	860	754-1613	Promoter of translation initiation factor gos2 gene from the Zea mays.
9	V-intervening sequences		41	1614-1654
10	I-1_ubi1-MAIZE	Zea mays	1013	1655-2667
11	V-intervening sequences		40	2668-2707
12	V-intervening sequences		24	2708-2731			Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® cloning system (attB1)
13	V-intervening-SYNTH	Synthetic	17	2732-2748
14	CS-m28-MAIZE	Zea mays	857	2749-3605	MADS-box m28 transcription factor gene.	Enhanced growth rate or yield	including 5' and 3' untranslated regions (UTRs; 60 bp and 41 bp respectively)
15	V-intervening-SYNTH	Synthetic	16	3606-3621
16	V-intervening sequences		24	3622-3645			Bacteriophage lambda integrase recombination site from the Invitrogen Gateway® cloning system (attB2)
17	V-intervening-SYNTH	Synthetic	14	3646-3659
18	T-pinII-SOLTU	Solanum tuberosum	308	3660-3967	3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence.
19	V-intervening sequences		30	3968-3997
20	V-intervening sequences		21	3998-4018			Bacteriophage lambda integrase recombination site (attB3)
21	V-intervening-SYNTH	Synthetic	73	4019-4091
22	V-loxP-BPP1	Bacteriophage P1	34	4092-4125			Bacteriophage P1 recombination site recognized by Cre recombinase (loxP)
23	V-intervening-SYNTH	Synthetic	19	4126-4144
24	P-ubi1-MAIZE	Zea mays	900	4145-5044	Promoter region of the polyubiquitin gene. Christensen et al. (1992). Plant Mol Biol, 18, 675-689
25	L-ubi-MAIZE	Zea mays	83	5045-5127
26	I-1_ubi1-MAIZE	Zea mays	1013	5128-6140
27	V-intervening sequences		76	6141-6216
28	V-intervening sequences		48	6169-6216			Flippase recombination target site from Saccharomyces cerevisiae (FRT1)
29	V-intervening-SYNTH	Synthetic	26	6217-6242
30	CS-pat-STRVR	Streptomyces viridochromogenes	552	6243-6794	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37	Glufosinate tolerance	Maize-optimized phosphinothricin acetyltransferase gene (mo-pat)
31	V-intervening sequences		7	6795-6801
32	T-pinII-SOLTU	Solanum tuberosum	311	6802-7112	3' untranslated region (3' UTR) of the proteinase inhibitor II gene; 0.31kb terminator sequence.
33	V-intervening sequences		21	7113-7133
34	V-intervening sequences		48	7134-7181			Modified flippase recombination target site derived from Saccharomyces cerevisiae (FRT87)
35	V-intervening-SYNTH	Synthetic	207	7182-7388
36	V-intervening-RHIRD	Agrobacterium tumefaciens	57	7389-7445
37	V-LB-RHIRD	Agrobacterium tumefaciens	25	7446-7470

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

patent documents Yes 7470

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	patent documents	Yes	7470

Detection Methods

16 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	3
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-P-Ubi-F/P-Ubi-R	element-specific	P-ubi1-MAIZE	Qualitative real-time PCR (TaqMan) method for detection of ubiquitin promoter of Zea mays (Debode et al., 2013)	in-house validation	none	1
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-EVE-ZM-034	event-specific	DP202216	Quantitative real-time PCR method for detection of maize event DP202216 (EU-RL GMFF GMOMETHODS database as of July 20, 2023)	ring trial validation	EU reference method	1
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-008	taxon-specific	Zea mays	Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-zSSIIb-3F/zSSIIb-4R	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)	unknown	unknown	1

Catalogue number	Source	Value	Type	Certified	Available
ERM-BF444a	JRC - CRM (ERM)	0 % (m/m)	dried powder	yes	yes
ERM-BF444b	JRC - CRM (ERM)	100 % (m/m)	dried powder	yes	yes

Citation	Publication year	Publishing authority	Publication type	Content types
Assessment of genetically modified maize DP202216 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA-GMO-NL-2019-159)	2024	EFSA GMO Panel	Scientific opinion EFSA GMO Panel	risk assessment document
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-034	2023	JRC	EU reference method protocol	detection
Application for authorisation in the European Union of DP202216 maize according to Regulation (EC) No 1829/2003 on genetically modified food and feed. EFSA-GMO-NL-2019-159 Part VII - Summary	2019	EFSA	application summary	application summary
Anderson JA, Brustkern S, Cong B, Deege L, Delaney B, Hong B, Lawit S, Mathesius C, Schmidt J, Wu J, Zhang J, Zimmermann C (2019) Evaluation of the history of safe use of the maize ZMM28 protein. J Agric Food Chem. 67(26):7466-7474	2019	Research group	peer-reviewed article	environment, safety

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference