GMO molecular characterization details

GMO detailed information

GMO:
Bt176
Alias:

Bt-176, CG 00256-176, 176
UID:
SYN-EV176-9
Tradename:

NaturGard KnockOut, Maximizer
Companies/Institutions:
Syngenta, Ciba Seeds
Species:
Zea mays
(maize, corn)
Traits:
EU authorisation:

No results found.

General Information

Inserts / Modification

Name:	insert1_Bt176
Comment:	The described gene cassette is probably present in at least four copies. Upon insertion of the transgene into the recipient maize genome the terminator of the gene cassette was lost. Therefore, the coding sequence CS-bar-STRHY is immediately followed by maize genomic DNA.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait
1	V-pUC_oriC-SYNTH	Synthetic	-
2	P-35S-CaMV	Cauliflower mosaic virus	-
3	CS-bar-STRHY	Streptomyces hygroscopicus	-	Phosphinothricin acetyltransferase gene derived from the common soil bacterium Streptomyces hygroscopicus a.k.a. bar gene shares 85 per cent homology at the amino acid level with the pat gene. Thompson et al. (1987) EMBO J. 6:2519-2523	Glufosinate tolerance

Related DNA Sequences:

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No
AJ783419	NCBI - GenBank	Yes	151	Zea mays sequence containing the junction region between 35SCaMV promoter and BAR gene, cultivar Bt176.
AJ878607	NCBI - GenBank	Yes	821	Synthetic construct Bt176 partial sequence for truncated BAR expression cassette and plant junction region.

Name:	insert2_Bt176
Comment:	The described order of genetic elements seems to be assured. However, the whole fragment might be present in multiple copies; information varies between two to five copies.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
2	I-9_pepc-MAIZE	Zea mays	-
3	CS-cry1Ab3_tr-BACTK	Bacillus thuringiensis ssp. Kurstaki	-	plant optimized gene coding for a truncated version Cry1Ab3 of B. thuringiensis ssp. Kurstaki HD-1 strain (aa sequence in the core protein showing the insecticidal activity of Cry1Ab protein remains unchanged); Geiser, M. et al. (1986). Gene, 48, 109-118	Lepidoptera resistance
4	P-pepc-MAIZE	Zea mays	-	Promoter of the peroxidase phosphoenolpyruvate carboxylase (PEPC) gene. Hudspeth and Grula (1989) Plant Mol Biol, 12(5):579-589
5	P-cdpk-MAIZE	Zea mays	-	Promoter of a calcium-dependent protein kinase gene. Estruch et al. (1994) Proc Natl Acad Sci USA, 91(19):8837-8841
6	CS-cry1Ab3_tr-BACTK	Bacillus thuringiensis ssp. Kurstaki	-	plant optimized gene coding for a truncated version Cry1Ab3 of B. thuringiensis ssp. Kurstaki HD-1 strain (aa sequence in the core protein showing the insecticidal activity of Cry1Ab protein remains unchanged); Geiser, M. et al. (1986). Gene, 48, 109-118	Lepidoptera resistance	truncated version of cry1Ab with 65% sequence identity to native form
7	I-9_pepc-MAIZE	Zea mays	-
8	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Transformation Vectors

Acc.No.	Reference	Sequence	Size (bp)	Annotation
AR494969	NCBI - GenBank	Yes	3468	Sequence 4 from patent US 6720488; full-length synthetic maize optimized Bt cry1A(b) gene.

Detection Methods

29 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-004	construct-specific	Bt176	Qualitative conventional PCR method for detection of maize event Bt176 (ISO 21569)	ring trial validation	ISO standard	3
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	3
QL-ELE-cry1A 4-5'/cry1A 4-3'	element-specific	CS-cry1Ab-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ab gene (Scholtens et al. 2013).	in-house validation	none	3
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	2
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	2
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	3
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	2
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QL-TAX-ZM-002	taxon-specific	Zea mays	Qualitative real-time PCR (SYBRGreen) method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-ZM-003	taxon-specific	Zea mays	Qualitative conventional PCR method for detection of the maize invertase gene (ISO 21569)	ring trial validation	ISO standard	1
QT-CON-00-007	construct-specific	B827, Bt176	Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (Shindo et al., 2002)	ring trial validation	ISO standard	3
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	3
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-003	element-specific	CS-cry1Ab-BACTU	Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570)	ring trial validation	ISO standard	3
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	3
QT-EVE-ZM-023	event-specific	Bt176	Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014).	in-house validation	EU reference method	3
QT-TAX-ZM-001	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene. Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-002	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize high-mobility-group A (hmgA) gene (ISO 21570). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	ISO standard	1
QT-TAX-ZM-003	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 (adh1) gene (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-004	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-ZM-005	taxon-specific	Zea mays	Quantitative PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of May 28, 2018).	ring trial validation	EU reference method	1
QT-TAX-ZM-006	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (ISO 21570)	ring trial validation	ISO standard	1
QT-TAX-ZM-008	taxon-specific	Zea mays	Quantitative real-time PCR method for detection of maize high-mobility-group (hmg) gene (EU-RL GMFF GMOMETHODS database as of July 20, 2023). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-ZM-011	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of the maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014)	ring trial validation	EU reference method	1
QT-TAX-ZM-014	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize alcohol dehydrogenase 1 gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-zSSIIb-3F/zSSIIb-4R	taxon-specific	Zea mays	Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)	unknown	unknown	1

Catalogue number	Source	Value	Type	Certified	Available
ERM-BF411a	JRC - CRM (ERM)	<0.014 % (m/m)	dried powder	yes	yes
ERM-BF411b	JRC - CRM (ERM)	0.1 % (m/m)	dried powder	yes	yes
ERM-BF411c	JRC - CRM (ERM)	0.5 % (m/m)	dried powder	yes	yes
ERM-BF411d	JRC - CRM (ERM)	1.0 % (m/m)	dried powder	yes	yes
ERM-BF411e	JRC - CRM (ERM)	2.0 % (m/m)	dried powder	yes	yes
ERM-BF411f	JRC - CRM (ERM)	5.0 % (m/m)	dried powder	yes	no
ERM-BF411g	JRC - CRM (ERM)	100 % (m/m)	dried powder	yes	yes
5211500701	Eurofins GeneScan	100 cp/µl	genomic DNA	no	no

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Related DNA Sequences

Detection Methods

Reference Materials

Authorisation

Literature reference