GMO molecular characterization details

GMO detailed information

GMO:
MLS 9124
Alias:
UID:
Tradename:
Companies/Institutions:
Metahelix Life Sciences
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

General Information

Inserts / Modification

Name:	insert_MLS 9124 (putative)
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the T-DNA of plasmid pMH82. Chhabra et al. (2014) describes a P-35S as promoter and a T-nos as terminator for nptII. In the patent, a P-nos and T-nos is described.
Map:

Genetic Elements:

Reference: patent documents

Elements:

Order	Name	Donor	Position	Definition	Trait	Comment
1	P-nos-RHIRD	Agrobacterium tumefaciens	-	Promoter region of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983). Nucleic Acids Res 11, 369-385; Fraley et al. (1983). Proc Nat L Acad Sci 80, 4803-4807
2	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
3	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807
4	V-nptII-ECOLX	Escherichia coli	-
5	P-35S-CaMV	Cauliflower mosaic virus	-			double 35S promoter
6	CS-cry1C-BACTU	Bacillus thuringiensis	-	plant-optimized gene incoding for the cry1C protein from Bacillus thuringiensis.
7	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
8	P-35S-CaMV	Cauliflower mosaic virus	-			double 35S promoter
9	CS-cry1C-BACTU	Bacillus thuringiensis	-	plant-optimized gene incoding for the cry1C protein from Bacillus thuringiensis.
10	T-35S-CaMV	Cauliflower mosaic virus	-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

patent documents No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	patent documents	No

Detection Methods

29 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-014	construct-specific	1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-023	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

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Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

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Literature reference