GMO molecular characterization details

GMO detailed information

GMO:
KWS20-1
Alias:
UID:
KB-KWS2Ø1-6
Tradename:
Companies/Institutions:
Bayer CropScience, KWS SAAT
Species:
Beta vulgaris
(sugar beet)
Traits:
EU authorisation:

no Reference materials could be found

General Information

Inserts / Modification

Name:	insert_KWS20-1
Comment:
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens		-
2	T-hsp20-MEDTR	Medicago truncatula		-	3' UTR sequence of the heat shock hsp20 gene from Medicago truncatula.
3	CS-pat-STRVR	Streptomyces viridochromogenes		-	Phosphinothricin acetyltransferase gene derived from Streptomyces viridochromogenes a.k.a. pat gene; shares 85 per cent homology at the amino acid level with the bar gene. Wohlleben et al. (1988) Gene 70:25-37	Glufosinate tolerance
4	P-cab1-ARATH	Arabidopsis thaliana		-	Promoter and leader sequence of chlorophyll a/b binding 1 gene (CAB1) from Arabidopsis thaliana. GenBank OK149192
5	T-guf2-MEDTR	Medicago truncatula		-	3' untranslated region (3' UTR) of an expressed Medicago truncatula gene of unknown function (guf). GenBank OK149195
6	CS-dmo-STEMA	Stenotrophomonas maltophilia		-	dicamba monooxygenase gene. Wang et al. (1997). Appl Environ Microbiol, 63, 1623-1626	Dicamba (2-methoxy-3,6-dichlorobenzoic acid) tolerance	insertion of a leucine immediately after the N-terminal methionine through cloning process
7	TP-rbcS-PEA	Pisum sativum		-			including the first 27 amino acids of the coding region of the rbcS gene
8	P-ubi1-CUCME	Cucumis melo		-	Promoter, leader, and intron sequence of the putative ubiquitin 1 gene from melon. GenBank OK149193
9	E-DMV	Dahlia mosaic virus		-	Full length transcript of the enhancer sequence derived from a Dalia mosaic virus promotor, Genbank accession EF513491, nucleotides 1 through 322. Flasinski (2020). Patent US20200224213A1.		contains part of the putative ORF of Dahlia mosaic virus
10	P-sams2-CUCME	Cucumis melo		-	Intron, 5'-terminal untranslated region, and promoter sequence of the SAMS2 gene encoding S-adenosyl-L-methionine synthase in melon. GenBank OK149194
11	TP-ctp2-ARATH	Arabidopsis thaliana		-
12	CS-CP4epsps-RHIRD	Agrobacterium tumefaciens ssp. CP4		-	5-enolpyruvylshikimate- 3-phosphate synthase gene. Steinruecken et al. (1980) Biochem Biophys Res Com. 94:1207-1212	Glyphosate tolerance	the second serine is modified to leucine compared to the wild type protein sequence
13	T-guf1-MEDTR	Medicago truncatula		-	3' untranslated region (3' UTR) of an expressed Medicago truncatula gene of unknown function (guf). GenBank MH931406
14	V-RB-RHIRD	Agrobacterium tumefaciens		-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

application documents No

Detection Methods

9 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-00-008	construct-specific	2904/1 kgs, ASR368, DBN9004, DBN9936, GT200, GT73, GTSB77, H7-1, J101, J163, KWS20-1, MON1445, MON1698, MON71200, MON71700, MON71800, MON80100, MON802, MON809, MON832, MON87411, MON87427, MON87429, MON87705, MON88017, MON88302, MON88913, MON89788, NK603, RBMT22-082, RBMT22-186, RBMT22-238, RBMT22-262, ZSR500, ZSR502, ZSR503	Qualitative real-time PCR (TaqMan) method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-epsps 1-5'/epsps 3-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4-epsps gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-epsps2 1-5'/epsps2 1-3'	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-Patf-n2/Patr-n2	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the pat gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-00-019	element-specific	CS-CP4epsps-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of CS-CP4epsps-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-021	element-specific	CS-pat-STRVR	Qualitative real-time PCR (SYBRGreen) method for detection of CS-pat-STRVR (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-025	element-specific	CS-pat-STRVR	Qualitative real-time PCR (TaqMan) method for detection of the synthetic phosphinothricin acetyltransferase (pat) gene (BVL L00.00-154). EU-RL GMFF GMOMETHODS database as of December 23, 2016.	ring trial validation	EU reference method	1
QT-ELE-00-002	element-specific	CS-pat-STRVR	Quantitative real-time PCR (TaqMan) method for detection of phosphinothricin N-acetyltransferase gene (Weighardt et al., 2004) (EURL GMFF GMOMETHODS database as of February 5, 2020)	ring trial validation	unknown	1
QT-TAX-BV-013	taxon-specific	Beta vulgaris	Quantitative real-time PCR (TaqMan) method for detection of sugar beet glutamine synthetase (GS2) gene (EU-RL GMFF GMOMETHODS database as of March 05, 2014)	ring trial validation	EU reference method	1

Authorisation

Literature reference