Description:
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GE-Low PPO Potato lines were developed using the CRISPR/Cas9 technology with two different methods that enable delivery of CRISPR/Cas9 elements into plant cells. Either by Agrobacterium-mediated transformation using a binary vector or by the Ribonucleoprotein (RNP) method. Each selected potato line was the result of either one of these methods.
The intended phenotype of GE-Low PPO Potato lines is reduced levels of polyphenol oxidase proteins, enzymes responsible for black spot, enzymatic darkening, and discoloration in potato tubers. CRISPR/Cas9 was used to generate one or more targeted DNA double-stranded breaks within the coding region of PPO gene(s). No external repair template was provided.
With either transformation method, the final selected lines do not contain any introduced DNA from the CRISPR/Cas9 system. The application document states that plants generated with the Agrobacterium-method were molecularly screened for the absence of all elements of the binary vector. In case of the RNP method, where no vector DNA was transferred, no screening was performed.
Source:
- USDA-APHIS letter of inquiry 20-168-01_air
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Links regarding to this GMO:
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USDA APHIS https://www.aphis.usda.gov/biotechnology/downloads/reg_loi/20-168-01_air_inquiry_cbidel.pdf
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Transformation / Modification technique:
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CRISPR/Cas
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