GMO molecular characterization details

GMO detailed information

GMO:
CZW-3
Alias:
UID:
SEM-ØCZW3-2
Tradename:
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Cucurbita pepo
(squash)
Traits:
EU authorisation:

no Reference materials could be found
No results found.
no Literature reference could be found

Inserts / Modification

Name:	insert_CZW-3
Comment:	Insert order indicates order of genetic elements as described for cassettes present in the construct. Order of cassettes between V-RB-RHIRD and V-LB-RHIRD is not clear.
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens		-
2	P-35S-CaMV	Cauliflower mosaic virus		-
3	CS-nptII-ECOLX	Escherichia coli		-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
4	T-35S-CaMV	Cauliflower mosaic virus		-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
5	P-35S-CaMV	Cauliflower mosaic virus		-
6	CS-cp-WMV	Watermelon mosaic virus		-	Coat protein gene from WMV2. Quemada et al. (1990). Journal of General Virology, 71, 1451-1460	Watermelon mosaic virus resistance
7	T-35S-CaMV	Cauliflower mosaic virus		-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
8	P-35S-CaMV	Cauliflower mosaic virus		-
9	CS-cp-ZYMV	Zucchini yellow mosaic virus		-	Coat protein gene from ZYMV. Quemada et al. (1990). Journal of General Virology, 71, 1451-1460	Zucchini yellow mosaic virus resistance
10	T-35S-CaMV	Cauliflower mosaic virus		-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
11	P-35S-CaMV	Cauliflower mosaic virus		-
12	CS-cp-CMV	Cucumber mosaic virus		-	Coat protein gene from CMV. Quemada et al. (1991). Phytopathology, 81, 794-802	Cucumber mosaic virus resistance
13	T-35S-CaMV	Cauliflower mosaic virus		-	3' untranslated region (3' UTR) of the 35S gene from Cauliflower mosaic virus. Gardner et al. (1981). Nucleic Acids Res, 9:2871-2888
14	V-RB-RHIRD	Agrobacterium tumefaciens		-

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Detection Methods

14 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-35S-F/nptII-R	construct-specific	23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010).	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-T-35S-F/T-35S-R	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (TaqMan) method for detection of the 35S terminator from Cauliflower mosaic virus (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-023	element-specific	T-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of T-35S pCAMBIA sequences (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1

Authorisation

Literature reference