GMO molecular characterization details

GMO detailed information

GMO:
27531
Alias:
UID:
SHD-27531-4
Tradename:
Companies/Institutions:
Florigene, Suntory
Species:
Dianthus caryophyllus
(carnation)
Traits:
EU authorisation:

no Reference materials could be found

Inserts / Modification

Name:	insert_27531
Comment:	Application submitted under directive 2001/18/EC - Summary notification file (SNIF) http://gmoinfo.jrc.ec.europa.eu/csnifs/C-NL-13-01.pdf
Map:

Genetic Elements:

Reference: application documents

Elements:

Order	Name	Donor	Size (bp)	Position	Definition	Trait	Comment
1	V-LB-RHIRD	Agrobacterium tumefaciens	697	1-697
2	V-MCS-SYNTH	Synthetic	8	698-705			Residual sequences (8 bp of polylinker) from E. coli vectors series pBluescript and pUC used in assembling transformation vector.
3	P-35S-CaMV	Cauliflower mosaic virus	191	706-896
4	L-cab-PETHY	Petunia hybrida	61	897-957
5	CS-SuRB-TOBAC	Nicotiana tabacum		958-	Acetohydroxy acid synthase gene (SuRB). Mazur et al. (1987). Plant Physiol 85, 1110-1117	Sulfonylurea tolerance
6	T-SuRB-TOBAC	Nicotiana tabacum		-4719	3' untranslated region (3' UTR) of the Nicotiana tabacum Acetohydroxy acid synthase gene. Mazur et al. (1987) Plant Physiol, 85(4): 1110-1117
7	V-MCS-SYNTH	Synthetic	266	4720-4985			Residual pBluescript and/or pUC series vector DNA used for making the construct. Cloning & ligation sites.
8	P-dfr-PETHY	Petunia hybrida		4986-	Promoter of the dihydroflavonol-4-reductase gene. Huits et al. (1994). Plant J, 6(3):295-310
9	CS-dfr-PETHY	Petunia hybrida		-	Dihydroflavonol-4-reductase gene. Beld et al. (1989). Plant Mol Biol, 13, 491-502	Colour modification
10	T-dfr-PETHY	Petunia hybrida		-9943	3' untranslated region (3' UTR) of the dihydroflavonol 4-reductase gene. Huits et a. (1994) The Plant J. 6(3):295-310
11	V-MCS-SYNTH	Synthetic	41	9944-9984			Residual pBluescript and/or pUC series vector DNA used for making the construct. Cloning & ligation sites.
12	P-chs-ANTMA	Antirrhinum majus	1158	9985-11142	Promoter of the chalcone synthase gene. Sommer et al. (1988). Mol Gen Genet, 211, 49-55
13	V-MCS-SYNTH	Synthetic	44	11143-11186			Residual pBluescript and/or pUC series vector DNA used for making the construct. Cloning & ligation sites.
14	CS-F3_5_H-VIOLA	Viola sp.	1777	11187-12963	Flavonoid 3',5'-hydroxylase (F3'5'H) gene from Viola sp.; is a cytochrome P450 type mono-oxygenase. Tanaka and Brugliera (2013). Philos Trans R Soc Lond B Biol Sci, 368(1612):20120432 doi: 10.1098/rstb.2012.0432. Patent WO2004020637A9	Colour modification
15	V-MCS-SYNTH	Synthetic	12	12964-12975			Residual pBluescript and/or pUC series vector DNA used for making the construct. Cloning & ligation sites.
16	T-D8-PETHY	Petunia hybrida	819	12976-13794	3' untranslated region (3' UTR) of the putative phospholipid transfer protein D8 from Petunia hybrida.
17	V-MCS-SYNTH	Synthetic	187	13795-13981			Residual pBluescript and/or pUC series vector DNA used for making the construct. Cloning & ligation sites.
18	V-RB-RHIRD	Agrobacterium tumefaciens	280	13982-14261

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

application documents No

Detection Methods

10 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	2
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-EVE-DC-005	event-specific	27531	Qualitative PCR method for detection of carnation event 27531 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2016/2050) (EURL GMFF GMOMETHODS database as of December 1, 2016)	in-house validation	EU reference method	3
QL-TAX-DC-001	taxon-specific	Dianthus caryophyllus	Qualitative conventional PCR method for detection of carnation anthocyanidin synthase (EURL GMFF GMOMETHODS database as of June 1, 2016).	in-house validation	EU reference method	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	2
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1

Authorisation

Literature reference