Description:
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Southern blot, PCR and sequence analyses indicated integration of two copies of the functional bialaphos resistance gene cassette (P-35S-CaMV > CS-pat-STRVR > T-35S-CaMV) of transformation vector pB2/35SacK in a head-to-tail configuration at a single genomic locus.
Between these two cassettes there are two non-functional fragments of a disrupted beta-lactamase gene (BSM-bla-ECOLX). The non-functional fragments are inserted in an inverted orientation to each other and do therefore not constitute a functional gene.
Source: Biotechnology Consultation on Food from GE Plant Varieties submitted under the U.S. Food and Drug Administration (FDA) “Statement of Policy: Foods Derived from New Plant Varieties" (the 1992 policy): Biotechnology Consultation Note to the File BNF No. 000055
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Links regarding to this GMO:
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BCH http://bch.cbd.int/database/record.shtml?documentid=14764,
EFSA application document http://registerofquestions.efsa.europa.eu/roqFrontend/questionDocumentsLoader?question=EFSA-Q-2005-174
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Transformation / Modification technique:
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biolistic method
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