GMO molecular characterization details

GMO detailed information

GMO:
MON1076
Alias:

MON89924, 1076, 89924
UID:
MON-89924-2
Tradename:
Companies/Institutions:
Bayer Crop Science (formerly Monsanto Company)
Species:
Gossypium hirsutum
(cotton)
Traits:
EU authorisation:

no Reference materials could be found
No results found.

Inserts / Modification

Name:	insert_MON1076
Comment:	complete and intact copy of sequence intended for integration from transformation vector PV-GHBK03
Map:

Genetic Elements:

Reference: BCH

Elements:

Order	Name	Donor	Position	Definition	Trait
1	P-34S FMV	Figwort mosaic virus	-	34S promoter derived from Figwort mosaic virus (FMV). Shepard et al. (1987) Phytopathology 77:1668-1673; Richins et al. (1987) Nucl Acids Res. 15:8451-8466; Gowda et al. (1989) J Cell Biochem. 13D (supplement):301
2	CS-cry1Ac-BACTU	Bacillus thuringiensis ssp. Kurstaki	-	Codon optimized gene or artificial gene incoding for (at least the insect-active portion of) the cry1Ac protein similar to the Bacillus thuringiensis var. kurstaki HD73. Adang et al. (1985) Gene 36:289-300; Fischhoff and Perlak (1996) U.S. patent 5500365	Lepidoptera resistance
3	T-rbcS_E9-PEA	Pisum sativum	-	3' untranslated region (3' UTR) f the rinulose-1,5-bisphosphate carboxylase, small subunit (rbcS E9) gene. Coruzzi et al. (1984) EMBO J. 3:1671-1679
4	P-35S-CaMV	Cauliflower mosaic virus	-
5	CS-nptII-ECOLX	Escherichia coli	-	Neomycin phosphotransferase II gene from the Eschrichia coli Tn5 transposon. Beck et al (1982). Gene, 19, 327-336	Kanamycin/neomycin resistance
6	T-nos-RHIRD	Agrobacterium tumefaciens	-	3' untranslated region (3' UTR) of the nopaline synthase gene from Agrobacterium tumefaciens T-DNA. Bevan et al. (1983) Nucleic Acids Res. 11: 369-385; Fraley et al. (1983) Proc Nat L Acad Sci. 80:4803-4807

Related DNA Sequences:

Acc.No. Reference Sequence Size (bp) Annotation

BCH No

Acc.No.	Reference	Sequence	Size (bp)	Annotation
	BCH	No

Detection Methods

32 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-35S-F/nptII-R	construct-specific	23-18-17, 23-198, 31807, 31808, 5345, 8338, ATBT04-06, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36, BT10 potato, BT12 potato, BT16 potato, BT17 potato, BT18 potato, BT23 potato, BT6 potato, BXN10211, CZW-3, EE-1, Event 1, FLAVR SAVR, MON1076, MON1445, MON15985, MON1698, MON531, MON757, MON80100, MON863, MON87460, SP951, SPBT02-7, pap1 GM Gypsophila	Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the nptII gene (Reiting, 2010).	in-house validation	none	1
QL-ELE-Cry1Ac-F(/R)-n4/Cry1AcR-n2	element-specific	CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the cry1Ac gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-PFMV 1-5'/PFMV 1-3'	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of P-FMV (Mano et al., 2009)	in-house validation	none	1
QL-ELE-P-FMV-F/P-FMV-R	element-specific	P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of the P-FMV promoter (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-004	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)	ring trial validation	ISO standard	1
QL-ELE-00-005	element-specific	P-Cauliflower mosaic virus	Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569)	ring trial validation	ISO standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-010	element-specific	E-FMV, P-34S FMV	Qualitative conventional PCR method for detection of Figwort mosaic virus 34S promoter (Pan et al., 2007)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	1
QL-ELE-00-012	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Qualitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (partim P-35S). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-015	element-specific	E-FMV, P-34S FMV	Qualitative real-time PCR (TaqMan) method for detection of Figwort mosaic virus 34S promoter (ISO/TS 21569-5).	ring trial validation	ISO standard	1
QL-ELE-00-016	element-specific	CS-cry1Ab-BACTU, CS-cry1Ab_1Ac_tr-SYNTH, CS-cry1Ac-BACTU	Qualitative real-time PCR (TaqMan) method for detection of the element coding for synthetic cry1Ab/Ac genes (ISO/TS 21569-6).	ring trial validation	ISO standard	1
QL-ELE-00-017	element-specific	P-Cauliflower mosaic virus	Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-024	element-specific	T-rbcS_E9-PEA	Qualitative real-time PCR (TaqMan) method for detection of the terminator of the pea ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) E9 gene (EU-RL GMFF GMOMETHODS database as of January 20, 2017).	ring trial validation	EU reference method	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-ELE-P35S 1-5'/P35S 1-3'	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)	ring trial validation	national standard	1
QT-ELE-00-001	element-specific	P-Cauliflower mosaic virus	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (Feinberg et al., 2005)	ring trial validation	unknown	1
QT-ELE-00-004	element-specific	E-35S-CaMV, P-Cauliflower mosaic virus, P-scp1-SYNTH	Quantitative real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter (ISO 21570 Annex B.1)	ring trial validation	ISO standard	1
QT-TAX-GH-015	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton fiber-specific acyl carrier protein (ACP1) gene (EU-RL GMFF GMOMETHODS database as of January 07, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-016	taxon-specific	Gossypium hirsutum	Quantitative PCR method for detection of putative cotton SAH7 protein gene (123 bp) (Mazzara et al., 2006). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-GH-018	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of January 17, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-019	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (Taqman) method for detection of cotton alcohol dehydrogenase C gene (EU-RL GMFF GMOMETHODS database as of November 1, 2018). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-020	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of cotton alcohol dehydrogenase C (adhC) gene (EU-RL GMFF GMOMETHODS database as of July 2020). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-GH-021	taxon-specific	Gossypium hirsutum	Quantitative real-time PCR (TaqMan) method for detection of putative cotton SAH7 protein gene (115bp) (EU-RL GMFF GMOMETHODS database as January 22, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1

Description:	Southern blot analysis indicated integration of one complete copy of the sequence intended for transfer from transformation vector PV-GHBK03 and one 1.6 kb incomplete and non-functional fragment which consists of a portion of CS-cry1Ac-BACTU and a portion of the 3'UTR.
Links regarding to this GMO:	BCH http://bch.cbd.int/database/record.shtml?documentid=14785
Transformation / Modification technique:	Agrobacterium-induced

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

Authorisation

Literature reference