Description:
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The Setaria viridis line 193-31 was created by CRISPR/Cas9 mediated mutagenesis.
The expressed CRISPR/Cas9 system targeted the coding region of the Setaria viridis homolog of the Zea mays ID1 gene and deactivated it. The construct used is described in the USDA-APHIS inquiry.
The expressed gRNA (RNA36003-2) was designed to target the second exon of the Setaria viridis ID1 homolog (Sevir.9G247100). This is a homolog of the ID1 gene that promotes flowering in maize. The deactivation of the homolog in Setaria viridris leads to delayed flowering time.
In the knock-out line 193-31, the CRISPR/Cas9 DNA construct was segregated away.
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Links regarding to this GMO:
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Donald Danforth Plant Science Center USDA-APHIS inquiry https://www.aphis.usda.gov/biotechnology/downloads/reg_loi/16-066-01_air_inquiry.pdf,
USDA-APHIS confirmation of non-regulation status https://www.aphis.usda.gov/biotechnology/downloads/reg_loi/16-066-01_air_response_signed.pdf
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Transformation / Modification technique:
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CRISPR/Cas
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