GMO molecular characterization details

GMO detailed information

GMO:
Ms1 x Rf2
Alias:

PGS2, PHY23
UID:
ACS- BNØØ4-7 x ACS-BNØØ2-5
Tradename:

InVigor
Companies/Institutions:
Bayer CropScience, Plant Genetic Systems
Species:
Brassica napus
(canola, oilseed rape, rapeseed)
Traits:
EU authorisation:

no Reference materials could be found

Detection Methods

35 results

Name	Type	Target	Description	Validation	Standardisation	Verification
QL-CON-PGS-bar-A2/PGS-bar-B2	construct-specific	Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)	Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13).	ring trial validation	national standard	1
QL-CON-00-014	construct-specific	1345-4, 16-0-1, 18-2-4, 35 1 N, 55-1, 5B, 63-1, A1-DFR Petunias, B tomato, C5, Cantaloupe A, Cantaloupe B, Da tomato, EH92-527-1, F tomato, F3'5'H GM petunia, FP967, GD743, GFM Cry1A, GK19, GS784, H421, HCN10, HLMT15-15, HLMT15-3, HLMT15-46, Huafan No 1, Huanong No. 1, INI-FMAPDF, KMD1, MLS 9124, MPS965, Ms1, NF872, NXI-1T, NXI-4T, NXI-6T, PG451, RBMT15-101, RBMT21-129, RBMT21-152, RBMT21-350, RL01-15, RL01-17, RM3-3, RM3-4, RM3-6, Rf1, Rf2, SEMT15-02, SEMT15-15, SGK321, SY233, T120-7, TAM66274, Topas 19/2, X17-2	Qualitative real-time PCR (TaqMan) method for detection of the junction between the nopaline synthase promoter (P-nos)and the neomycin phosphotransferase II gene (nptII) (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-AgroBS1 F/AgroBS1 R	element-specific	V-Flanking Ti-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the nopaline type aka Agroborder I. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	1
QL-ELE-AgroBS2 F/AgroBS2 R	element-specific	V-Flanking Ti-ocs-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of the Agrobacterium tumefaciens Ti plasmids of the octopine type aka Agroborder II. Part of the qualitative triplex real-time PCR (TaqMan) method for detection of Ti plasmid (AgroBorder) sequences and a sequence of the Cassava vein mosaic virus promoter pat construct (P-CsVMV-pat) for screening for components of genetically modified organisms in food (Official Collection of Methods according to § 64 LFGB, L 00.00-176)	ring trial validation	national standard	1
QL-ELE-bar 2-5'/bar 2-3'	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009)	in-house validation	none	1
QL-ELE-B'nase-F-n4/BNaseR-n3	element-specific	CS-barnase	Qualitative real-time PCR (TaqMan) method for detection of the barnase gene (Scholtens et al. 2013).	in-house validation	none	1
QL-ELE-BstarF-n2 /BstarR-n2	element-specific	CS-barstar	Qualitative real-time PCR (TaqMan) method for detection of the barstar gene (Xu et al., 2006)	in-house validation	none	1
QL-ELE-npt 1-5'/npt 1-3'	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative real-time PCR (TaqMan) method for detection of the nptII gene (Scholtens et al., 2013).	in-house validation	none	1
QL-ELE-P-nos-F/P-nos-R	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-SSuAra-F/P-SSuAra-R	element-specific	P-rbcS-ARATH	Qualitative real-time PCR (TaqMan) method for detection of SSUAra promoter (ribulose 1.5-biphoshate carboxylase small subunit) of Arabidopsis thaliana (Debode et al., 2013)	in-house validation	none	1
QL-ELE-P-TA29-F/P-TA29-R	element-specific	P-ta29-TOBAC	Qualitative real-time PCR (TaqMan) method for detection of P-TA29 tapetal specific promoter of Nicotiana tabacum (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-g7-F/T-g7-R	element-specific	T-orf23-RHIRD, T-transcript 7	Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-T-OCS-F/T-OCS-R	element-specific	T-ocs-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of octopine synthase terminator of Agrobacterium tumefaciens (Debode et al., 2013)	in-house validation	none	1
QL-ELE-00-002	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of neomycin phosphotransferase II gene (ISO 21569)	ring trial validation	ISO standard	1
QL-ELE-00-003	element-specific	CS-nptII-ECOLX, V-nptII-ECOLX	Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001)	ring trial validation	national standard	1
QL-ELE-00-006	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Official Collection of Methods according to § 64 LFGB, L 00.00-31)	ring trial validation	national standard	1
QL-ELE-00-007	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)	ring trial validation	national standard	1
QL-ELE-00-008	element-specific	P-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase promoter (ISO/TS 21569-4)	ring trial validation	ISO standard	1
QL-ELE-00-009	element-specific	T-nos-RHIRD	Qualitative conventional PCR method for detection of nopaline synthase terminator (Lipp et al.,2001)	ring trial validation	ISO standard	1
QL-ELE-00-011	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (ISO 21569 AMD1).	ring trial validation	ISO standard	2
QL-ELE-00-013	element-specific	T-nos-RHIRD	Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos). Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)	ring trial validation	ISO standard	1
QL-ELE-00-014	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (ISO 21569 AMD1)	ring trial validation	ISO standard	2
QL-ELE-00-018	element-specific	T-nos-RHIRD	Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-022	element-specific	CS-bar-STRHY	Qualitative real-time PCR (SYBRGreen) method for detection of CS-bar-STRHY (Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-ELE-00-026	element-specific	CS-bar-STRHY	Qualitative real-time PCR (TaqMan) method for detection of the element coding for phosphinothricin N-acetyltransferase (CS-bar-STRHY) gene from Streptomyces hygroscopicus (partim bar) (Debode et al., 2016).	ring trial validation	EU reference method	1
QL-TAX-BN-003	taxon-specific	Brassica napus	Qualitative real-time PCR (SYBRGreen) method for detection of oilseed rape cruciferin A gene(Barbau-Piednoir et al., 2014)	ring trial validation	unknown	1
QL-TAX-BN-004	taxon-specific	Brassica napus	Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007).	ring trial validation	unknown	1
QT-ELE-NOS ter 2-5' /NOS ter 2-3'	element-specific	T-nos-RHIRD	Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)	ring trial validation	none	1
QT-EVE-BN-005	event-specific	Ms1	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Ms1 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)	in-house validation	EU reference method	1
QT-EVE-BN-007	event-specific	Rf2	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Rf2 (verified by the EU-RL GMFF in the context of Commission Decision 2007/306/EC)	in-house validation	unknown	1
QT-TAX-BN-001	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape acyl-ACP thioesterase (FatA) gene (EU-RL GMFF GMOMETHODS database as of January 23, 2014) Estimated number of target copies per haploid genome (Jacchia et al. 2018): 1	ring trial validation	EU reference method	1
QT-TAX-BN-002	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin storage protein (EU-RL GMFF GMOMETHODS database as of January 27, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 4	ring trial validation	EU reference method	1
QT-TAX-BN-003	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A gene (Jacchia et al. 2019) (EU-RL GMFF GMOMETHODS database as of January 15, 2020)	ring trial validation	EU reference method	1
QT-TAX-BN-012	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	ring trial validation	EU reference method	1
QT-TAX-FatA primer1/FatA primer2	taxon-specific	Brassica napus	Quantitative real-time PCR (TaqMan) method for the detection of oilseed rape (Monsanto, 2004, A recommended procedure for real-time quantitative TaqMan PCR for Roundup Ready canola RT73). Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2	in-house validation	none	1

Description:	For Reference Materials, please consult the materials mentioned under the separate GMO events.
Links regarding to this GMO:
Transformation / Modification technique:	conventional crossing

Citation	Publication year	Publishing authority	Publication type	Content types
Commission Implementing Decision (EU) 2022/736 of 11 May 2022 amending Decisions 2007/305/EC, 2007/306/EC and 2007/307/EC as regards the tolerance period for traces of Ms1xRf1 (ACS-BNØØ4-7 x ACS-BNØØ1-4) hybrid oilseed rape, Ms1xRf2 (ACS- BNØØ4-7 x ACS-BNØØ2-5) hybrid oilseed rape and Topas 19/2 (ACS-BNØØ7-1) oilseed rape, as well as their derived products	2022	EU	EU Commission Decision	decision document
Commission Implementing Decision (EU) 2019/1562 of 16 September 2019 amending Decisions 2007/305/EC, 2007/306/EC and 2007/307/EC as regards the tolerance period for traces of Ms1×Rf1 (ACS-BNØØ4-7×ACS-BNØØ1-4) hybrid oilseed rape, Ms1×Rf2 (ACS-BNØØ4-7 × ACS-BNØØ2-5) hybrid oilseed rape and Topas 19/2 (ACS-BNØØ7-1) oilseed rape, as well as their derived products	2019	EU	EU Commission Decision	decision document
Commission Implementing Decision (EU) 2016/2268 of 14 December 2016 amending Decisions 2007/305/EC, 2007/306/EC and 2007/307/EC as regards the tolerance period for traces of Ms1×Rf1 (ACS-BNØØ4-7×ACS-BNØØ1-4) hybrid oilseed rape, Ms1×Rf2 (ACS-BNØØ4-7 × ACS-BNØØ2-5) hybrid oilseed rape and Topas 19/2 (ACS-BNØØ7-1) oilseed rape, as well as their derived products	2016	EU	EU Commission Decision	decision document
PCR detection of the pSSUAra-bar construct for screening of genetically modified oilseed rape lines - construct-specific method. BVL Official Method Collection G 30.40-13 Online Publication (2015)	2015		other document	detection
Commission Implementing Decision of 3 February 2012 amending Decisions 2007/305/EC, 2007/306/EC and 2007/307/EC as regards the tolerance period for traces of Ms1xRf1 (ACS-BNØØ4-7xACS-BNØØ1-4) hybrid oilseed rape, Ms1xRf2 (ACS-BNØØ4-7 x ACS-BNØØ2-5) hybrid oilseed rape and Topas 19/2 (ACS-BNØØ7-1) oilseed rape, as well as of their derived products, (2012/69/EU)	2012	EU	EU Commission Decision	decision document
Commission Decision of 25 April 2007 on the withdrawal from the market of Ms1xRf2 (ACS-BNØØ4-7xACS-BNØØ2-5) hybrid oilseed rape and its derived products (notified under document number C(2007) 1806), (2007/306/EC)	2007	EU	EU Commission Decision	decision document
Commission Decision of 6 June 1997 concerning the placing on the market of genetically modified swede-rape (Brassica napus L. oleifera Metzg. MS1, RF2), pursuant to Council Directive 90/220/EEC, (97/393/EC)	1997	EU	EU Commission Decision	decision document

European GMO Initiative for a Unified Database System

GMO detailed information

GMO:

Alias:

UID:

Tradename:

Companies/Institutions:

Species:

Traits:

EU authorisation:

General Information

Links regarding to this GMO:

Inserts / Modification

Transformation Vectors

Detection Methods

Reference Materials

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Literature reference