Description:
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EH92-527-1 was developed through Agrobacterium-mediated transformation of the potato cultivar Prevalent with the binary vector pHoxwG.
The intended consequence of the genetic modification in EH92-527-1 is to inhibit of the expression of granule bound starch synthase protein (GBSS) responsible for amylose biosynthesis. As a result, the starch produced has little or no amylose and consists of amylopectin (branched starch), which modifies the physical properties of the starch. A gene conferring kanamycin resistance (nptII) was used as a selectable marker.
Molecular analysis shows that potato EH92-527-1 contains two partial copies of the DNA fragment, i.e. the insert, including the flanking region, was duplicated in reverse orientation and joined tail-to-tail. This is present at a single locus in the nuclear genome of the GM plant.
EH92-527-1 was confirmed to be free of Agrobacterium-derived vector backbone DNA. Molecular analysis indicated that the DNA insert was stable over years.
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Links regarding to this GMO:
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Transformation / Modification technique:
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Agrobacterium-induced
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